摘要
试验旨在探究饲粮中添加不同水平牛至精油对芦花鸡肉品质及肉质相关基因表达的影响。选取49日龄健康芦花鸡120只体重(1478.75±85.81)g,随机分为4组,每组3个重复,每个重复10只鸡,各组饲粮分别添加0(Q8组)、50(QO50组)、100(QO100组)和150 mg/kg(QO150组)的牛至精油,试验期75 d(预试期3 d,正试期72 d)。饲喂结束后屠宰并测定肉品质及肉质相关基因的表达量。结果表明:1)与Q8组相比,藜麦饲粮中添加牛至精油对芦花鸡胸肌LRP1基因有一定影响,显著提高QO100组LRP1基因mRNA表达量(P<0.05)。2)与Q8组相比,藜麦饲粮中添加牛至精油显著降低了QO50组腿肌组织中CAPN1基因mRNA表达量(P<0.05)。3)胸肌组织中,LRP1基因的表达水平与失水率、滴水损失、剪切力呈显著正相关(P<0.05);CAST基因的表达水平与滴水损失、剪切力呈显著正相关(P<0.05);CAPN1基因的表达水平与剪切力呈显著负相关(P<0.05)。4)腿肌组织中,LRP1基因表达水平与剪切力呈显著正相关(P<0.05);CAST基因的表达水平与失水率和滴水损失呈显著正相关(P<0.05);CAPN1基因的表达水平与滴水损失呈极显著负相关(P<0.01),与剪切力呈显著负相关(P<0.05)。由此可见,饲粮中添加牛至精油对肌肉组织中LRP1基因、CAPN1基因的表达量有一定影响,且LRP1、CAST、CAPN1基因与嫩度相关肉品质指标存在显著相关性。
This experiment was conducted to investigate the effects of different levels of dietary oregano essential oil on quality and meat quality related gene expression of Luhua chicken meat.A total of 12049-day-old healthy Luhua chickens(body weight 1478.75±85.81 g)were randomly divided into 4 groups with 3 replicates per group and 10 chickens per replicate.The diets of each group were supplemented with 0(Q8 group),50(QO50 group),100(QO100 group)and 150 mg/kg oregano essential oil(QO150 group),respectively.The experiment lasted for 75 days(3 days for pre-test and 72 days for formal test).At the end of feeding,meat quality and the expression of meat quality related genes were measured.The results showed 1)compared with group Q8,quinoa diet supplemented with oregano essential oil had a certain effect on LRP1 gene in breast muscle of Luhua chicken,and significantly increased the mRNA expression of LRP1 gene in group QO100(P<0.05).2)Compared with group Q8,quinoa diet supplemented with oregano essential oil significantly decreased the mRNA expression level of CAPN1 gene in leg muscle of QO50 group(P<0.05).3)The expression level of LRP1 gene was significantly positively correlated with water loss rate,drip loss and shear force in thoracic muscle tissue(P<0.05).CAST gene expression level was positively correlated with drip loss and shear force(P<0.05).The expression level of CAPN1 gene was negatively correlated with shear force(P<0.05).4)LRP1 gene expression level was positively correlated with shear force in leg muscle tissue(P<0.05).The expression level of CAST gene was positively correlated with water loss rate and drip loss(P<0.05).The expression level of CAPN1 gene was negatively correlated with drip loss(P<0.01)and shear force(P<0.05).In conclusion,dietary oregano essential oil has a certain effect on the expression levels of LRP1 gene and CAPN1 gene in muscle tissue,and LRP1,CAST and CAPN1 genes are significantly correlated with meat quality indexes related to tenderness.
作者
吴涛
江小帆
杨发荣
魏玉明
陈国顺
蔡原
焦婷
赵生国
WU Tao;JIANG Xiaofan;YANG Farong;WEI Yuming;CHEN Guoshun;CAI Yuan;JIAO Ting;ZHAO Shenguo(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China;Dingxi Vocational and Technical College,Dingxi,Lanzhou,Gansu 730500;Institute of Livestock,Grass and Green Agriculture,Gansu Academy of Agricultural Sciences,Lanzhou,Gansu 730070,China;College of Pratacultural Science,Gansu Agricultural University,Lanzhou,Gansu 730070,China)
出处
《家畜生态学报》
北大核心
2025年第5期21-27,共7页
Journal of Domestic Animal Ecology
基金
中央引导地方科技发展专项(特色藜麦产业培育及科技扶贫模式推广)
甘肃省科协学会助力精准扶贫项目(甘科协20190002)
甘肃省现代农业科技支撑体系区域创新中心重点科技项目(2019GAAS51)
兰州市科技计划项目(2020-1-10,2019-4-52)。
