摘要
随着全球食品安全问题日益严重,开发快速且灵敏的检测技术显得尤为重要。核酸等温扩增技术因其高效、快速和操作简便的特点,在食品安全检测领域中的重要性日益凸显。该文系统阐释了环介导等温扩增技术、滚环扩增技术、重组酶聚合酶扩增技术、酶促重组等温扩增技术、等温链置换技术、核酸多臂引物和环优化技术等核酸等温扩增技术在食品安全检测领域的应用现状,涵盖主要类型、基本原理、具体应用、与其他技术结合的优势以及未来发展趋势。通过分析近年来的研究成果,旨在为食品安全检测领域提供新的思路和参考,进一步促进相关技术的发展与应用。
As global food safety issues become more severe,the development of rapid and sensitive detection technologies has become increasingly critical.Characterized by efficiency,speed,and simplicity,nucleic acid isothermal amplification technologies are playing an increasingly vital role in the field of food safety detection.This paper systematically explores the applications of various nucleic acid isothermal amplification methods in food safety detection,including loop-mediated isothermal amplification(LAMP),rolling circle amplification(RCA),recombinase polymerase amplification(RPA),enzymatic recombinase amplification(ERA),strand displacement amplification(SDA),and nucleic acid multi-arm primers and circular optimization technologies.The discussion encompasses the main types,basic principles,specific applications,advantages of combining these methods with other technologies,and future development trends.By analyzing recent research advancements,this study aims to provide new perspectives and references in the field of food safety detection.It further seeks to promote the development and application of these emerging technologies.
作者
郭雨湄
贾振军
刘瑞
GUO Yumei;JIA Zhenjun;LIU Rui(School of Investigation,People’s Public Security University of China,Beijing 100038,China)
出处
《食品与发酵工业》
北大核心
2025年第11期435-448,共14页
Food and Fermentation Industries
基金
中央高校基本科研业务费专项资金资助项目(2022JKF435)。
关键词
环介导等温扩增技术
滚环扩增技术
重组酶聚合酶扩增技术
酶促重组等温扩增技术
等温链置换技术
核酸多臂引物和环优化技术
食品安全检测
loop-mediated isothermal amplification
rolling circle amplification
recombinase polymerase amplification
enzymatic recombinase amplification
isothermal strand displacement polymerization reaction
amplifying DNA with multiarm priming and looping optimization of nucleic acid
food safety detection
