摘要
采用水热法快速制备Fe_3O_4纳米粒子,并通过表面氨基化与金纳米粒子自组装构建金磁微粒(Fe_3O_4@Au).表征Fe_3O_4@Au理化性质,并优化Fe_3O_4@Au对牛血清蛋白的固定体系.实验结果表明,所制备的金纳米粒子平均粒径达到(7.8±0.4)nm,氨基化的磁性材料可以固载金纳米粒子,金磁微粒饱和磁化强度为61 emu/g;金磁微粒对牛血清蛋白的固定化体系为金磁微粒添加量0.1 g,固定化温度50℃,固定化时间10 min,且平均固定量为5.189 mg/g.
In this paper,the Fe_3O_4 nanoparticles were prepared using hydrothermal method.The gold magnetic particles（ Fe_3O_4@ Au） were formed by the Fe_3O_4 nanoparticles combined with gold nanoparticles using layer-by-layer method based on the surface amino groups.The preparation processes and properties of the assembling-type Fe_3O_4@ Au were described in this paper. And also the system of bovine serum albumin was established by single factor analysis and orthogonal design analysis. The results showed that the average particle size of gold nanoparticles was（ 7. 8 ± 0. 4） nm. The magnetic materials functionalized with amino groups could be loaded with gold nanoparticles,and the saturation magnetization of Fe_3O_4@Au was 61 emu/g. The optimal process was determined that the quantity of gold magnetic particles was 0. 1 g,and the temperature was 50 ℃,the time of immobilization was 10 min.The highest immobilize quantity was 5. 189 mg/g under the condition of the optimization experiment.
出处
《哈尔滨商业大学学报（自然科学版）》
CAS
2017年第4期450-454,468,共6页
Journal of Harbin University of Commerce：Natural Sciences Edition
关键词
金磁微粒
牛血清蛋白
固定化
优化工艺
gold magnetic particles
bovine serum albumen
immobilization
optimal process
