摘要
针对寒区低温条件下采用沼液养藻进行资源化存在能耗高、效率低的问题,筛选了可快速适应15℃低温环境的6株微藻。考察了其对沼液氨氮(ammonia nitrogen,NH_(4)^(+)-N)耐受力与污染物去除效果,并探索了微藻对低温抑制的解除机制。结果表明,6株微藻在15℃下可耐受400 mg·L^(-1)NH_(4)^(+)-N,生物量最高可达204.67~223.79 mg·L^(-1)。叶绿素a、叶绿素b、类胡萝卜素浓度为4.77~5.84、1.73~2.23、1.46~2.09 mg·L^(-1)。NH_(4)^(+)-N、总磷(total phosphorus,TP)去除率分别为44.09%~47.02%与6.63%~15.60%。培养10 d后,衣藻(W-4)生物量最高(223.79 mg·L^(-1));NH_(4)^(+)-N(47.02%)、TP(15.60%)去除效果最好;总叶绿素、叶绿素a、叶绿素b、类胡萝卜素浓度分别达8.07、5.84、2.23与2.09 mg·L^(-1),比极地裂丝藻和普通衣藻分别高17.09%~55.81%、7.66%~9.49%、264.49%~437.93%、35.89%~42.88%、46.97%~47.87%、13.48%~31.27%、31.21%~42.73%。较高的类胡萝卜素含量表明,沼液中高营养盐浓度可能削弱了微藻在低温条件下细胞内生成的氧化应激(ROS)的伤害,提高了藻体生物量。所筛藻种具有耐低温、耐高氨氮的双重功能,且生长周期较短,为寒区微藻养殖及沼液资源化提供了宝贵的种质资源。
In addressing challenges related to high energy consumption and low efficiency in utilizing microalgae for biogas slurry resource utilization under cold conditions,six microalgae strains adaptable to 15℃ were screened.Their tolerance to NH_(4)^(+)-N and pollutant removal from biogas slurry were investigated.The release mechanisms of the low-temperature inhibition were also explored.At 15℃,the six strains could tolerant 400 mg∙L^(−1) NH_(4)^(+)-N,and the biomass concentration reached 204.67~223.79 mg∙L^(−1).Chlorophyll a,chlorophyll b,and carotenoid concentrations ranged from 4.77~5.84,1.73~2.23,and 1.46~2.09 mg∙L^(−1),respectively.NH_(4)^(+)-N and TP removal ranged from 44.09%~47.02%and 6.63%~15.60%,respectively.After 10 days’cultivation,Chlamydomonas(W-4)exhibited the highest biomass(223.79 mg∙L^(−1))and NH_(4)^(+)-N(47.02%)and TP(15.60%)removal.The total chlorophyll,chlorophyll a,chlorophyll b,and carotenoids concentration were 8.07,5.84,2.23,and 2.09 mg∙L^(−1),respectively,which increased by 17.09%~55.81%,7.66%~9.49%,264.49%~437.93%,35.89%~42.88%,46.97%~47.87%,13.48%~31.27%,and 31.21%~42.73%compared with polar Stichococcus antarticus and common Chlamydomonas,respectively.The enhanced carotenoid content in the screened microalgae suggested that the mitigation of the oxidative stress(ROS)damage in microalgae cells at low temperatures by the high nutrient levels might be the main reason for promoting the microalgal biomass concentration.Results revealed that the screened strains exhibited high tolerance to the low-temperature and high NH_(4)^(+)-N concentration.The growth period was shorter than the other previous species,which providing valuable genetic resources for cold region in microalgae cultivation and biogas slurry utilization.
作者
王翠翠
卢海凤
刘德钊
张光明
司哺春
蒋伟忠
李保明
WANG Cuicui;LU Haifeng;Liu Dezhao;Zhang Guangming;SI Buchun;JIANG Weizhong;LI Baoming(College of Water Resources and Civil Engineering,China Agricultural University Beijing 100083,China;Key Laboratory of Agricultural Engineering in Structure and Environment,Ministry of Agriculture and Rural Affairs of the PRC,Beijing 100083,China;College of Biosystems Engineering and Food Science,Zhejiang University,Hangzhou 150030,China;College of Energy and Environmental Engineering,Hebei University of Technology,Tianjin 300131,China)
出处
《环境工程学报》
北大核心
2025年第4期772-783,共12页
Chinese Journal of Environmental Engineering
基金
国家自然科学基金区域创新发展联合基金项目(U21A20162)。
关键词
低温微藻
寒区种质资源
猪粪沼液
耐寒抗逆性
low-temperature microalgae
germplasm resources in cold regions
pig manure biogas slurry
cold and stress resistance
