摘要
为明确河北省保定市部分县区辣椒炭疽病病原菌种类,以望都、顺平和安新县采集的辣椒炭疽病病果为材料,利用组织分离法获得炭疽病病原菌36株,通过形态学和分子生物学鉴定,确定31株为平头炭疽菌Colletotrichum truncatum,5株为斯高威尔炭疽菌C.scovillei,分离比例分别为86.1%和13.9%。采用菌丝生长速率法测定了5种杀菌剂对C.scovillei BD38和C.truncatum BD94的室内抑菌效果。结果表明,咯菌腈对这2个菌株抑菌效果最好,EC_(50)分别为0.0120μg/mL和0.0142μg/mL;多菌灵和苯醚甲环唑对C.scovillei表现较好的抑制效果,EC_(50)分别为0.2905μg/mL和0.8450μg/mL,嘧菌酯和啶酰菌胺抑制效果稍差。多菌灵和苯醚甲环唑同样对C.truncatum效果较好,EC_(50)分别为0.1972μg/mL和0.2012μg/mL,嘧菌酯和啶酰菌胺对C.truncatum抑制效果较差(EC_(50)分别为1.3529μg/mL和2.9769μg/mL)。本试验结果明确了保定地区辣椒炭疽病的病原,为生产中防治由C.truncatum、C.scovillei引起的辣椒炭疽病提供理论依据和用药参考。
To identify the causal agent species of anthracnose on Capsicum annuum L.in Baoding,Hebei province,diseased pepper fruits were collected from Wangdu,Shunping and Anxin.Thirty-six strains were isolated and identified as Colletotrichum truncatum and C.scovillei,with isolation rate of 86.1%and 13.9%,respectively.Five fungicides were employed to assay inhibition efficiency in the laboratory against two species of C.scovillei BD38 and C.truncatum BD94.Among them,fludioxonil showed the best inhibition effect on these two strains,with EC_(50)values of 0.0120μg/mL and 0.0142μg/mL,respectively.Carbendazim and difenoconazole showed good inhibition effect on C.scovillei,with EC_(50)values of 0.2905 to 0.8450μg/mL.These two fungicides also showed better effect on C.truncatum,with EC_(50)values of 0.1972μg/mL and 0.2012μg/mL,respectively.The inhibition effect of azoxystrobin and boscalid to C.truncatum were relatively lower(1.3529μg/mL and 2.9769μg/mL,respectively).The research identified the pathogen of pepper anthracnose in Baoding,and provided theoretical basis and fungicide recommendation for the prevention and control of pepper anthracnose caused by C.truncatum and C.scovillei.
作者
朱亚楠
王冰雪
戴祎卓
杨军玉
胡同乐
张娜
杨文香
ZHU Yanan;WANG Bingxue;DAI Yizhuo;YANG Junyu;HU Tongle;ZHANG Na;YANG Wenxiang(College of Plant Protection,Hebei Agricultural University,Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province,Baoding 071000,China)
出处
《植物保护》
北大核心
2025年第3期339-344,353,共7页
Plant Protection
基金
河北省现代农业产业技术体系建设专项(HBCT202140207)
武汉市园林植物炭疽病绿色防控技术研究与应用(WHGF2023A04)。
关键词
辣椒炭疽病
病原鉴定
杀菌剂
室内毒力测定
chili anthracnose disease
pathogen identification
fungicide
in vitro toxicity assay
