摘要
背景:前期研究通过2次烧结法将玻璃材料渗透入5Y-PSZ超透氧化锆中,制备了能保持高透明性且抗弯强度较高的5Y-PSZ-YGI梯度玻璃分级超透氧化锆材料。目的:评估5Y-PSZ-YGI梯度玻璃分级超透氧化锆材料的体外生物相容性。方法:①通过2次烧结将玻璃材料渗透入5Y-PSZ超透氧化锆中,制备5Y-PSZ-YGI梯度玻璃分级氧化锆。将5Y-PSZ-YGI梯度玻璃分级超透氧化锆(或5Y-PSZ超透氧化锆、3Y-TZP透明氧化锆)置入含体积分数10%胎牛血清DMEM培养基中12,24,72 h,培养基与试样表面积比为3 mL/cm^(2),得到12,24,72 h材料浸提液。②将小鼠成纤维细胞L929培养24 h后弃原培养基,分7组培养:对照组更换为含体积分数10%胎牛血清DMEM培养基,其余6组分别更换为3Y-TZP透明氧化锆24 h浸提液、5Y-PSZ超透氧化锆24 h浸提液、5Y-PSZ-YGI梯度玻璃分级超透氧化锆24 h浸提液、3Y-TZP透明氧化锆72 h浸提液、5Y-PSZ超透氧化锆72 h浸提液、5Y-PSZ-YGI梯度玻璃分级超透氧化锆72 h浸提液。培养1,3,5 d后,显微镜下观察细胞生长,通过CCK-8检测得到细胞增殖率,判定细胞毒性。③将人抗凝血分别与5Y-PSZ-YGI梯度玻璃分级超透氧化锆、5Y-PSZ超透氧化锆、3Y-TZP透明氧化锆混合,0.5 h后检测溶血率。将人抗凝血分别与3Y-TZP透明氧化锆12 h浸提液、5Y-PSZ超透氧化锆12 h浸提液、5Y-PSZ-YGI梯度玻璃分级超透氧化锆12 h浸提液混合,0.5 h后检测溶血率。结果与结论:①显微镜下可见,随着培养时间的延长,各组细胞数量增加,并且各实验组细胞形态与对照组基本一致。3Y-TZP透明氧化锆24 h浸提液组培养第1天的细胞毒性分级0级,其余实验组在各个时间段的细胞毒性分级均为1级。②无论是材料还是材料浸提液均未引起明显的溶血反应,溶血率均低于5%。③结果表明,5Y-PSZ-YGI梯度玻璃分级超透氧化锆对小鼠成纤维细胞L929生长增殖无明显影响,并且不会与人血发生溶血反应,具有良好的体外生物相容性。
BACKGROUND:In previous studies,glass materials were infiltrated into 5Y-PSZ ultra-translucent zirconia by a double sintering method to prepare 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia materials that can maintain high transparency and high flexural strength. OBJECTIVE: To evaluate the in vitro biocompatibility of 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia materials. METHODS: (1) Glass materials were infiltrated into 5Y-PSZ ultra-translucent zirconia by double sintering to prepare 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia. 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia (or 5Y-PSZ ultra-translucent zirconia, 3Y-TZP transparent zirconia) was placed in DMEM culture medium containing 10% fetal bovine serum for 12, 24 and 72 hours, and the surface area ratio of culture medium to sample was 3 mL/cm2, and the 12-, 24- and 72-hour material extracts were obtained. (2) After culturing mouse fibroblast L929 for 24 hours, the original culture medium was discarded and divided into 7 groups for culture: the control group was replaced with DMEM culture medium containing 10% fetal bovine serum by volume, and the other 6 groups were replaced with 24-hour extract of 3Y-TZP transparent zirconia, 24-hour extract of 5Y-PSZ ultra-translucent zirconia, 24-hour extract of 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia, 72-hour extract of 3Y-TZP transparent zirconia, 72-hour extract of 5Y-PSZ ultra-translucent zirconia, and 72- hour extract of 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia. After 1, 3, and 5 days of culture, cell growth was observed under a microscope, and the cell proliferation rate was obtained by CCK-8 assay to determine cytotoxicity. (3) Human anticoagulated blood was mixed with 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia, 5Y-PSZ ultra-translucent zirconia, and 3Y-TZP transparent zirconia, and the hemolysis rate was detected after 0.5 hours. Human anticoagulated blood was mixed with 12-hour extract of 3Y-TZP transparent zirconia, 12-hour extract of 5Y-PSZ ultra-translucent zirconia, and 12-hour extract of 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia, and the hemolysis rate was detected after 0.5 hours. RESULTS AND CONCLUSION: (1) Under the microscope, it could be seen that the number of cells in each group increased with the extension of culture time, and the cell morphology of each experimental group was basically the same as that of the control group. The cytotoxicity grade of the 24-hour extract of 3Y-TZP transparent zirconia group on the first day of culture was grade 0, and the cytotoxicity grade of the other experimental groups at each time period was grade 1. (2) Neither the material nor the material extract caused obvious hemolytic reaction, and the hemolytic rate was less than 5%. (3) The results showed that 5Y-PSZ-YGI graded glass infiltrated ultra-translucent zirconia had no significant effect on the growth and proliferation of mouse fibroblasts L929, and did not cause hemolytic reaction with human blood, and had good in vitro biocompatibility.
作者
张其娅
童伊翔
杨世姣
张宇梦
邓凌
吴玮
解瑶
廖健
毛岭
Zhang Qiya;Tong Yixiang;Yang Shijiao;Zhang Yumeng;Deng Ling;Wu Wei;Xie Yao;Liao Jian;Mao Ling(School/Hospital of Stomatology,Guizhou Medical University,Guiyang 550004,Guizhou Province,China;Guizhou Provincial People’s Hospital,Guiyang 550002,Guizhou Province,China;Pingba District People's Hospital,Anshun 561199,Guizhou Province,China)
出处
《中国组织工程研究》
北大核心
2026年第2期443-450,共8页
Chinese Journal of Tissue Engineering Research
基金
贵州省卫生健康委科学技术基金项目(gzwjkj2021-1-342),项目负责人:毛岭
贵州医科大学博士启动资金(校博合J字[2020]11号),项目负责人:毛岭。
关键词
梯度玻璃分级超透氧化锆
生物相容性
超透氧化锆
透明氧化锆
细胞毒性
溶血率
生物材料
工程化口腔材料
graded glass infiltrated ultra-translucent zirconia
biocompatibility
ultra-translucent zirconia
transparent zirconia
cytotoxicity
hemolysis rate
biomaterial
engineered oral material
