摘要
实验室前期通过转录组测序技术在黑果枸杞和红果枸杞中筛选出了叶绿素b代谢候选差异基因NYC1,为研究其表达调控分子机制,分别分离出两者NYC1基因的启动子序列并进行生物信息学分析。结果表明,黑果枸杞NYC1基因启动子的核心启动子区在-659~-609 bp之间,不含有CpG岛,而红果枸杞NYC1基因启动子在-5009~-4959 bp之间,为核心启动子区的概率最高,含有CpG岛;相比于黑果枸杞,通过序列比对发现红果枸杞NYC1基因启动子序列存在3处插入片段。本研究结果初步揭示了黑果枸杞和红果枸杞NYC1基因启动子序列中存在差异,为进一步研究NYC1基因表达调控的分子机制提供了新的线索。
The research team previously screened the chlorophyll b metabolism candidate differential gene NYC1 in black and red wolfberry through transcriptome sequencing.In order to preliminarily explore the regulatory molecular mechanism among them,we isolated the promoter sequence of NYC1 gene in black and red wolfberry respectively and carried bioinformatics analysis.The result showed that core promoter region of NYC1 gene promoter in black wolfberry was between-659~-609 bp and not contain CpG islands,while the core promoter region of NYC1 gene promoter in red wolfberry was between-5009~-4959 bp and contain CpG islands.Compared with black wolfberry,the promoter sequence of NYC1 gene in red wolfberry had three insert fragments,and there were multiple cis-acting elements in the three insert fragments.It preliminarily revealed the differences in the promoter sequences of NYC1 gene between black and red wolfberry,and provided a new clue for further studying the molecular mechanism of NYC1 gene expression regulation.
作者
刘峰
刘光瑞
李云
曹东
刘宝龙
宗渊
Liu Feng;Liu Guangrui;Li Yun;Cao Dong;Liu Baolong;Zong Yuan(School of Life Science,Qinghai Normal University,Xining,810008;Key Laboratory of Crop Molecular Breeding of Qinghai Province,Chinese Academy of Sciences,Xining,810001)
出处
《分子植物育种》
北大核心
2025年第7期2364-2371,共8页
Molecular Plant Breeding
基金
青海省基础研究计划项目(2021-ZJ-955Q)资助。
