摘要
通过测定缺钼与施钼处理冬小麦品种叶绿素含量的相对比值(SPAD值),研究了不同时期小麦叶绿素、类胡萝卜素含量、蛋白酶活性及叶绿素a、b比值(Ch la/Ch lb)的变化,以探讨冬小麦缺钼黄化的原因。结果表明,34个冬小麦品种叶绿素SPAD值缺钼处理显著低于施钼处理,说明冬小麦黄化的原因是缺钼引起冬小麦叶绿素含量下降;播种后27和57 d(低温时期)的两个冬小麦品种叶绿素含量缺钼处理显著低于施钼处理,播种后135 d(高温时期)的两个冬小麦品种缺钼和施钼处理叶绿素含量没有显著差异,进一步说明低温时期缺钼易引起冬小麦叶绿素含量下降,从而导致冬小麦黄化现象的发生。缺钼时类胡萝卜素含量同叶绿素含量一样显著下降,并与之在不同时期呈现一致的变化趋势。缺钼与施钼处理间蛋白酶活性无显著差异,表明缺钼时叶绿素含量的下降不是分解加剧的结果,而可能是由于叶绿素合成受阻所致。两个冬小麦品种缺钼和施钼处理Ch la/Ch lb较为恒定,无显著差异。然而播种后27、57和84 d(温度较低时期)Ch la/Ch lb比值显著高于播种后135 d(温度较高时期),表明叶绿素a向叶绿素b的转化受低温影响,而与钼营养的丰缺无关。
The mechanism of yellowish caused by low temperature and low available Mo is not clear. SPAD values of relative chlorophyll in CK （without Mo application） were significantly lower than in Mo application in 34 winter wheat cultivars. Chlorophyll and carotenoid content were found significantly lower in CK than in Mo application in 27 and 57 d, but not in 135 d, which showed that Mo deficiency caused the decrease of chlorophyll under low temperature. There was no significant difference between CK and Mo application in the activity of proteinase, thus disclosed that the decrease of chlorophyll was not caused by the breaking down of chlorophyll. The ratios of Ch1a / Ch1b in Mo application were obviously higher in 27 d, 57 d after sowing for No Mo application （cold stge）, but there was no difference between the Mo and No Mo treatments in 135 d after sowing, this indicated that the transformation from ch1a to ch1b was not affected by Mo deficiency as did by low temperature. Thus indicated that Mo deficiency did not cause the breaking down of chlorophyll, and it might inhibited the biosynthesis of chlorophyll, but having no effect on the transformation of ch1a to ch1b.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2006年第2期113-116,共4页
Journal of Triticeae Crops
基金
国家自然科学基金项目(39770428
30070431)
农业部九五重点项目(95农-18-04)
关键词
冬小麦
叶绿素
蛋白酶
钼
Winter wheat
Chlorophyll
Proteinase
Molybadenum
