摘要
在环境可控的人工光植物工厂中,研究了23.328 mol/(m^(2)·d)日累积光量下LED红蓝绿光质的3种光照模式,即常规光照(18 h光照/6 h黑暗,红光∶蓝光∶绿光=280∶70∶10)、连续光照(24 h光照/0 h黑暗,红光∶蓝光∶绿光=210∶52.5∶7.5)和3种光质交替光照(24 h光照/0 h黑暗,红蓝光∶绿光=525∶15;红绿光∶蓝光=435∶105;绿蓝光∶红光=120∶420)对紫花苜蓿产量品质和生理活性及氮代谢的影响。结果表明,紫花苜蓿是连续光照耐受型植物,与常规光照相比,连续光照下紫花苜蓿形态健康、产量未减,且可溶性糖、淀粉含量提高,但光合色素含量、气孔导度和Fv/Fm降低,没有累积过量H_(2)O_(2)和丙二醛。数据表明,红蓝光-绿光交替促进了叶片延展,红绿光-蓝光交替促进了株高增长,绿蓝光-红光交替使初花期提前。氮代谢方面,硝态氮含量在连续和绿蓝光-红光交替显著降低,氨态氮含量在红绿光-蓝光交替显著升高,硝酸还原酶活性在红蓝光-绿光交替显著降低,亚硝酸还原酶活性在红绿光-蓝光交替显著降低(P<0.05)。连续光照和交替光照抑制了氮代谢酶活性,但提高了紫花苜蓿可溶性蛋白、游离氨基酸和粗蛋白含量。综上所述,连续光照和交替光照模式适用于人工光植物工厂中紫花苜蓿的生产。
In this research,effects of three light modes of LED red,blue,and green light spectrum at 23.328 mol/(m^(2)·d)DLI(conventional light with 18 h/6 h,red∶blue∶green=280∶70∶10;continuous light with 24 h/0 h,red∶blue∶green=210∶52.5∶7.5;and alternating light with three light spectra:24 h/0 h,red∶blue∶green=525∶15;red-green light∶blue light=435∶105;green and blue light∶red light=120∶420)on yield quality,physiological activity,and nitrogen metabolism were determined in an controlled environment plant factory with artificial light.The results demonstrated the adaptability of alfalfa to continuous light.Compared with conventional lighting,healthy morphology and maintained yield were observed in alfalfa under continuous lighting,along with increased soluble sugar and starch content.However,decreases occurred in photosynthetic pigment content,stomatal conductance,and Fv/Fm,without excessive accumulating of H_(2)O_(2) and malondialdehyde.Further data showed that the alternation of red/bluegreen light promoted leaf expansion,the alternation of red/green-blue light promoted stem elongation,and the alternation of green/blue-red light shortened the flowering time.In terms of nitrogen metabolism,nitrate nitrogen content was significantly reduced under continuous and green/blue-red light alternation,while ammonium nitrogen content was significantly increased under red/green-blue light alternation.Activity of nitrate reductase was significantly reduced under red/blue-green light alternation,while red/greenblue light alternation significantly reduced nitrite reductase activity.The activity of nitrogen metabolism enzymes was inhibited by continuous and alternating lighting,whereas the content of soluble protein,free amino acids,and crude protein in alfalfa were increased.In summary,continuous lighting and alternating lighting patterns are suitable for the production of alfalfa in plant factory with artificial light.
作者
陈艳琦
林坤明
刘文科
CHEN Yanqi;LIN Kunming;LIU Wenke(Institute of Environment and Sustainable Development in Agriculture,Chinese Academy of Agricultural Sciences/Key Laboratory of Energy Conservation and Waste Management of Agricultural Structures,Ministry of Agriculture and Rural Affairs,Beijing 100081,China)
出处
《中国草地学报》
北大核心
2025年第5期78-88,共11页
Chinese Journal of Grassland
基金
中国农业科学院科技创新工程(CAAS-IEDA-T07)。
关键词
紫花苜蓿
植物工厂
连续光照
交替光照
生理活性
氮代谢
Alfalfa
Plant factory
Continuous light
Alternating light
Physiological activity
Nitrogen metabolism
