摘要
【目的】探究外源褪黑素对草地早熟禾(Poa pratensis)抗盐性的调节作用。【方法】以草地早熟禾品种帝国为试验材料,测定浸种和外施褪黑素2种方法对种子萌发特性的影响,采用叶施和根施不同浓度褪黑素溶液处理幼苗,研究其对草地早熟禾生长和生理特性的影响。【结果】浸种和外施褪黑素处理分别在其浓度为50、200μmol/L时对盐胁迫下的草地早熟禾种子萌发效果最好;当褪黑素浓度为100、200μmol/L时能够显著促进盐胁迫下草地早熟禾幼苗和根系的生长,积累幼苗干物质量和相对含水量,增加幼苗叶绿素含量,降低幼苗相对电导率和丙二醛含量,使得幼苗脯氨酸、可溶性糖和可溶性蛋白含量上升,并且在该浓度下叶施处理的效果更佳。【结论】浓度为100、200μmol/L的褪黑素溶液可以有效缓解盐胁迫对草地早熟禾幼苗的伤害,提高草地早熟禾的抗盐性。
【Objective】To investigate the regulatory effect of exogenous melatonin on Poa pratensis under salt stress.【Method】This experiment used the Poa pratensis‘Empire’,as the material.The effects of seed soaking and external application of melatonin on seed germination characteristics under salt stress were studied.In addition,different concentrations of melatonin were applied to the leaves and roots of seedlings to evaluate their effects on the growth and physiological characteristics of Poa pratensis under salt stress.【Result】The results showed that soaking and external application of melatonin treatment had the best germination effect on Poa pratensis seeds under salt stress at concentrations of 50μmol/Land 200μmol/L respectively.At concentrations of 100μmol/L and 200μmol/L,melantoninsignificantly promoted the growth of Poa pratensis seedlings and roots under salt stress,enhanced dry matter accumulation and relative water content,increased chlorophyll content,reduced relative conductivity and malondialdehyde(MDA)content,and elevated the contents of proline,soluble sugars,and soluble protein content of seedlings.Leaf application treatment within this concentration range was the most effective.【Conclusion】In summary,exogenous melatonin concentration of 100μmol/L and 200μmol/L melatonin solution could effectively alleviate the damage of salt stress to Poa pratensis seedlings,improving the salt resistance and growth performance of Poa praten-sis.
作者
张玉娟
崔林雨
张佳能
马晖玲
董文科
ZHANG Yu-juan;CUI Lin-yu;ZHANG Jia-neng;MA Hui-ling;DONG Wen-ke(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosys-tem Sustainability,Lanzhou 730070,China)
出处
《草原与草坪》
2025年第2期196-205,共10页
Grassland and Turf
基金
国家自然科学基金项目(32201457)
草业生态系统教育部重点实验室项目(甘肃农业大学)开放课题(KLGE202210)。
关键词
褪黑素
草地早熟禾
抗盐性
萌发特性
生长和生理指标
综合分析
melatonin
Poa pratensis
salt resistance
germination characteristics
growth and physiological index
comprehensive analysis
