摘要
探究玉米不同组织微环境与内生菌多样性的关系,对筛选玉米相合性促生菌或生防菌等功能微生物具有重要意义。采用高通量测序技术研究玉米不同器官组织内生细菌和真菌群落多样性,结果表明,玉米内生细菌群落由31个门,93个纲,192个目,340个科,404个属组成。变形菌门和厚壁菌门为优势细菌门,毛螺菌属、根瘤菌属、肠杆菌属及鞘氨醇单胞菌属为优势细菌属,且部分内生细菌表现出不同程度的器官偏好性,表明部分细菌种群存在组织微环境内生专一性。对比各器官组织优势细菌属,根部独有的细菌属是细球菌属和绒毛杆菌属;籽粒中独有的细菌属是葡萄球菌属、绒毛杆菌属、代尔夫特菌属、短波单胞菌属、链球菌属和狭窄梭菌属;叶中独有的细菌群主要是假单胞菌属。玉米根、茎、叶微生物群落组成具有相似性,但与籽粒差异较大。玉米内生真菌群落由12个门,37个纲,84个目,187个科,404个属组成。子囊菌门、担子菌门和壶菌门为优势真菌门,被孢霉属和镰刀菌属为优势真菌属,罗兹菌门是根部独有的优势真菌群。玉米茎、叶和籽粒器官组织间真菌群落组成差异较小,但与根部差异较大。综上,玉米器官组织对内生菌的分布具有较大的影响,不同器官组织内生细菌群落组成差异显著,内生真菌群落组成差异不明显。
The relationship between the microenvironment of different tissues of maize and the diversity of endophytic bacteria is of great significance for screening functional microorganisms,such as compatible growth-promoting bacteria or biocontrol bacteria.In this study,high-throughput sequencing technology was used to investigate the diversity of endophytic bacterial and fungal communities in various maize organs and tissues.The results showed that the maize endophytic bacterial community comprised 31 phyla,93 classes,192 orders,340 families,and 404 genera.Proteobacteria and Firmicutes were the dominant bacterial phyla,and Lachnospira,Rhizobium,Enterobacter,and Sphingomonas were the dominant bacterial genera.Some endophytic bacteria were more abundant in some organs than in others,indicating that some bacterial populations had endogenous specificity for particular tissue microenvironments.Comparing the dominant bacterial genera among various organs and tissues,the unique bacterial taxa in the roots were Micrococcus and uncultured_bacterium_f_Muribaculaceae.The unique bacterial taxa in the kernels were Staphylococcus,uncultured_bacterium_f_Muribaculaceae,Delftia,Brevundimonas,Streptococcus,and Clostridium_sensu_stricto_1.The unique bacterial group in the leaves was mainly Pseudomonas,and no unique bacterial genus was detected in the stems.Beta diversity analyses showed that the microbial community composition was similar in the roots,stems,and leaves of maize,but different in the grain.The endophytic fungal community of maize was composed of 12 phyla,37 classes,84 orders,187 families,and 404 genera.Ascomycota,Basidiomycota and Chytridiomycota were the dominant fungal phyla,Mortierella and Fusarium were the dominant fungal genera,and Rozellomycota was the unique dominant fungal group in the roots.Beta diversity analyses showed that there was little difference in fungal community composition among stems,leaves,and grain of maize,but it was significantly different in the root.In summary,the distribution of endophytic bacteria differs among maize organs and tissues.Compared with the endophytic fungal community,the endophytic bacterial community showed wider variations among the different organs and tissues.
作者
董晓慧
师尚礼
尹国丽
陈三冬
巩海强
刘林波
Xiao-hui DONG;Shang-li SHI;Guo-li YIN;San-dong CHEN;Hai-qiang GONG;Lin-bo LIU(College of Pratacultural Science,Gansu Agricultural University,Key Laboratory of Pratacultural Ecosystem,Ministry of Education,Pratacultural Engineering Laboratory of Gansu Province,The Sino-U.S.Centers for Grazingland Ecosystem Sustainability,Lanzhou 730070,China)
出处
《草业学报》
北大核心
2025年第5期130-145,共16页
Acta Prataculturae Sinica
基金
2024年度农业类省级技术创新引导计划-东西部科技协作专项全株青贮玉米“种+贮+喂”一体化技术集成及推广应用资助。
关键词
玉米
组织器官
内生细菌
内生真菌
多样性
maize
tissue and organs
endophytic bacteria
endophytic fungi
diversity
