摘要
采用20种不同培养基分离水葫芦内牛细菌，利用细菌脂肪酸鉴定技术对水葫芦的内生细菌进行鉴定，研究了水葫芦内生细菌的种类及群落结构。结果表明，共得到32个属56株内生细菌，种类最多的是微杆菌属（Microbacterium），共有9种细菌。其次为假单胞菌属（Pseudomonas），共有7种细菌。芽孢杆菌属（Bacillus）有5种细菌。其余23个属均有1种。利用优势度指数分析各培养基分离到内生细菌的多样性，10号葡萄糖、酵母、淀粉琼脂培养基优势度指数为0．1607，分离得到内生细菌种类最多为9种；其次是4号根瘤菌培养基和11号NA培养基的优势度指数为0．1250，分离得到的内生菌种类均为7种；16号黄豆芽汁培养基（pH7．2～7．4）和20号醋酸菌培养基，优势度指数为0．0893，分离获得内生细菌均为5种。
Endophyte bacteria were isolated from the plant tissues of Eichhornia crassipe （ Mart. ）Solms by using 20 types of cultural media. The collected 56 bacterial isolates were identified with bacterial fatty-acid identification system to study the population structure of endophyte bacteria inside E. crassipe. The endophyte bacteria were classified into 32 genera, while most belonged to Microbacterium with 9 isolates, Pseudomonas with 7 isolates and Bacillus with 5 isolatesa as well as the other 23 genus had only 1 isolate respectively. Study diversity of endiophyte bacterica on culture medium using dominance index, result showed dominance index of glucose, yeast, starch and agar culture medium is 0.160 7, endophyte bacteria is 9 species. Dominance index of Rhizobiumv culture medium and NA culture medium was 0.125 0, had 7 isolate respectively. Next was soybean sprouts juice culture medium （pH 7.2-7.4） and Acetobacter Aceti cuhure medium,dominance index was 0.089 3, endophyte bacteria was 5 species.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2008年第6期2423-2429,共7页
Journal of Agro-Environment Science
基金
福建省发改委重大项目(闽发高技[2005]1061号)
福建省财政专项-福建省农业科学院科技创新团队建设基金(STIF-Y03)
关键词
水葫芦
内生细菌
Eichhornia crassipe （ Mart. ）Sohns
endophyte bacteria
