摘要
目的 了解广东地区近十年水禽源沙门菌的流行情况及耐药现状,指导广东地区水禽源沙门病防控。方法 采用药敏纸片法对2013—2023年从广东水禽主要养殖区238个水禽养殖场分离到的314株水禽源沙门菌进行血清学鉴定和药物敏感性试验。结果 314株沙门菌分属B、D、C1、E1群9种血清型,其中优势血清型为鼠伤寒沙门菌;实验菌株对β内酰胺类的青霉素、阿莫西林、头孢拉定、头孢唑林,磺胺类磺胺二甲嘧啶和四环素类的四环素耐药率最高,耐药率为73.57%~89.49%;对氨基糖苷类的阿米卡星、庆大霉素、卡那霉素及喹诺酮类的诺氟沙星最为敏感,敏感率均超50%,其中280株沙门菌对6类抗菌药物表现多重耐药,5重耐药以上高达60.83%,相关性分析结果显示氟苯尼考跟庆大霉素、阿莫西林跟青霉素的相关性最高(r均为0.650),其次是庆大霉素跟卡那霉素(r=0.620)。结论 广东地区水禽源沙门菌耐药普遍严重,应加强对水禽病原检测,合理使用抗生素以防耐药菌传播。
This study was aimed at understanding the prevalence and drug resistance status of Salmonella of waterfowl origin in the Guangdong region in the past decade,to guide prevention and control efforts.The drug-sensitive paper slide method was used to conduct drug susceptibility testing on 314 waterfowl-originating Salmonella strains isolated from 238 waterfowl farms in the Guangdong region from 2013 to 2023.The isolated Salmonella strains were most resistant to penicillin,amoxicillin,cefradine,and cefazolin in theβ-lactam group;sulphadoxine dimethylpyrimidine in the sulphonamide group;and tetracycline in the tetracycline group.The resistance rates ranged from 73.57%to 89.49%.The highest sensitivity was observed to amikacin,gentamicin,and kanamycin in the aminoglycoside group,and norfloxacin in the quinolone group,with susceptibility rates all exceeding 50%.The 280 strains of Salmonella showed multi-drug resistance to six classes of antimicrobial drugs and high resistance(as much as 60.83%)to five drug classes.Correlation analysis revealed the highest correlations for florfenicol with gentamicin,and for amoxicillin with penicillin(r=0.650 for both),followed by gentamicin with kanamycin(r=0.620).Salmonella resistance in waterfowl in Guangdong Province was generally severe and showed a complex pattern of drug resistance.Detection of waterfowl pathogens should be strengthened to prevent the spread of drug-resistant bacteria and support rational use of antibiotics.This work provides a reference for Salmonella prevention and control in waterfowl farms.
作者
李宛珈
林寅盛
刘敏芳
薛文昌
朱婉君
陈济铛
张济培
LI Wan-jia;LIN Yin-sheng;LIU Min-fang;XUE Wen-chang;ZHU Wan-jun;CHEN Ji-dang;ZHANG Ji-pei(Foshan University,Foshan 528231,China;Guangdong Science and Trade Vocational College,Guangzhou 510430,China;Foshan Tianmu Biotechnology Co.,Ltd,Foshan 528231,China)
出处
《中国人兽共患病学报》
北大核心
2025年第3期297-303,共7页
Chinese Journal of Zoonoses
基金
2项专利权及水禽常见病原检测与多发病高效防控技术许可实施合同(No.BKH20902801)
佛山万牧洲生物有限公司技术开发项目(No.KH23279)
广东科贸职业学院一般项目(No.GDKM2023-11)。
关键词
沙门菌
血清分型
耐药表型
水禽
多重耐药
Salmonella
serotyping
resistance phenotypes
waterfowl
multi-drug resistanc
