摘要
探究骨碎补总黄酮(total flavonoids of rhizoma drynariae,TFRD)对去卵巢骨质疏松大鼠子宫内膜损伤和细胞凋亡的影响及可能机制。卵巢摘除术建立绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)大鼠模型。将SD大鼠随机分为4组:假手术组、模型组、TFRD治疗组、阳性药物(戊酸雌二醇片)组,后2组于术后第7天开始每天灌胃108 mg/(kg·d)TFRD或0.21 mg/(kg·d)戊酸雌二醇片,连续处理12周。H-E染色观察各组大鼠股骨和子宫组织病理学形态;TUNEL染色评估大鼠子宫内膜细胞凋亡情况;Western blotting检测大鼠子宫组织凋亡因子caspase-3的表达;ELISA检测大鼠子宫组织炎性因子IL-6、TNF-α和雌激素雌二醇(estradiol,E2)含量;免疫组化检测大鼠子宫组织雌激素受体α(estrogen receptor alpha,ESR1)表达。结果显示,相较假手术组,模型组大鼠股骨组织病理损伤加重,子宫出现病理变化,子宫内膜细胞凋亡增加,caspase-3蛋白表达及IL-6、TNF-α含量显著上升(均P<0.01),E2含量和ESR1表达显著下降(均P<0.01);相较模型组,TFRD治疗组及阳性药物组大鼠股骨组织和子宫内膜病理学改变减轻,子宫内膜细胞凋亡减少,caspase-3蛋白表达,IL-6、TNF-α含量均显著下降(均P<0.01),E2含量和ESR1表达显著增加(均P<0.01)。由此,TFRD可有效缓解PMOP大鼠子宫内膜损伤及细胞凋亡,其机制可能与其调控E2,抑制炎性因子IL-6、TNF-α表达有关。
The aim of this study was to explore the effect and possible mechanism of total flavonoids of rhizomadrynariae(TFRD)on endometrial damage and cell apoptosis in ovariectomized osteoporotic rats.Postmenopausal osteoporosis(PMOP)rat model was established by ovariectomy.The SD rats were randomly divided into 4 groups:the sham-operation group,the model group,the TFRD treatment group,and the positive drug(estradiol valerate)group.The latter two groups were daily gavaged with 108 mg/(kg·d)TFRD or 0.21 mg/(kg·d)estradiol valerate tablet from the 7th day after operation and treated for 12 weeks.H-E staining was used to observe the pathological morphology of rat femur and uterus tissues.Apoptosis of endometrial cells was evaluated by TUNEL staining.Western blotting was used to detect caspase-3 expression in uterus.ELISA was used to quantify levels of IL-6,TNF-α,and estradiol(E2)in the uterus tissues.Estrogen receptor alpha(ESR1)expression of uterus was detected by immunohistochemistry.Compared to the sham-operation group,the model group showed pathological injury of femur tissues and pathological changes in the uterus tissues.Apoptosis of endometrial cells was increased,along with elevated expressions of caspase-3,IL-6,and TNF-α,while the E2 and ESR1 expressions were decreased(all with P<0.01).Compared to the model group,the TFRD,and positive drug treatment groups exhibited improved pathological changes in rat femur tissues and uterus.Apoptosis of endometrial cells and the protein levels of caspase-3,IL-6,and TNF-αwere significantly decreased,while the expression levels of E2 and ESR1 were increased(all with P<0.01).Therefore,TFRD can effectively alleviate endometrial injury and apoptosis in PMOP rats,and the mechanism may involve the regulation of E2 and the inhibition of the expression of inflammatory factors IL-6 and TNF-α.
作者
方虹
龙凤
鲁林
FANG Hong;LONG Feng;LU Lin(Department of Gynecology,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430000,China;Department of Orthopedics,Wuhan Hospital of Traditional Chinese Medicine,Wuhan 430010,China)
出处
《现代免疫学》
2025年第2期118-124,共7页
Current Immunology
基金
湖北省自然科学基金创新发展联合基金项目(2022CFD150)
湖北省中医药管理局中医药科研项目(ZY2023F009)
湖北省时珍人才工程科研项目(鄂卫函[2024]256号)。
关键词
骨碎补总黄酮
绝经后骨质疏松症
子宫内膜
雌二醇
炎症反应
total flavonoids of rhizomadrynariae
postmenopausal osteoporosis
endometrium
estradiol
inflammation
