摘要
目的 探讨不等渗条件下肝细胞水化状态改变对某些代谢的影响。方法 动态观察培养鼠肝细胞 [3H]亮氨酸、[3H]TdR掺入量 ,肝细胞膜Na+ K+ ATP酶及上清液中ALT、AST、LDH活性。结果 培养12 0min ,与等渗组比较 ,高渗组肝细胞 [3H]亮氨酸和 [3H ]TdR掺入量分别减少 72 .99%和 6 5 .70 % (P <0 .0 0 1) ,低渗组减少 15 .94%和 10 .41% (P >0 .0 5 )。高渗组肝细胞膜Na+ K+ ATP酶活性增高 ,低渗组降低 ,与等渗组比较差异显著 (P <0 .0 5与P <0 .0 1)。肝细胞膜Na+ K+ ATP酶活性与 [3H ]掺入量在低渗组呈非常显著负相关 ,高渗组呈非常显著正相关 (P <0 .0 0 1)。培养上清液ALT、AST、LDH活性在正常范围内波动。结论 肝细胞水化状态可作为影响肝细胞代谢的触发因素 ,而Na+ K+ ATP酶活性改变可能是这一状态下细胞代谢调节机制之一。
Objective To observe certain metabolic changes induced by alterations of hepatocellular hydration state under aniso osmotic condition. Methods Incorporating rate of [ 3H] leucine into protein and that of [ 3H]TdR into DNA were measured and the activity of Na +/K + ATPase of hepatocellular membrane and that of ALT, AST and LDH in the supernatant of cell culture observed dynamically. Results After the cells were cultured for 120 min, the incorporating rates of [ 3H] leucine and [ 3H]TdR in the hepatocytes of hyper osmotic group were significantly inhibited as compared with the normo osmotic and hypo osmotic groups ( P <0.01 and 0.05). These two rates were closely correlated in all the 3 groups ( P <0.001). The activity of Na +/K + ATPase in the hyper osmotic group was markedly increased but that in the hypo osmotic group decreased as compared with that in the normo osmotic group ( P <0.05 and 0.01). The two incorporating rates in the hypo osmotic group were negatively but those in the hyper osmotic group positively correlated to the activity of Na +/K + ATPase. The activity of ALT, AST and LDH in the supernatant of cell culture was within the normal range. Conclusion The changes in hepatocellular hydration state may be one of the physiological factors to modulate hepatocellular metabolism, in which the activity of Na +/K + ATPase of hepatocellular membrane might regulate the levels of protein and DNA synthesis under this condition.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第5期490-492,共3页
Journal of Third Military Medical University
