摘要
番茄褐色皱纹果病毒(tomato brown rugose fruit virus,ToBRFV)近年来在全球番茄产区快速发生蔓延,造成严重的经济损失。云南建水番茄上出现疑似ToBRFV感染的症状,并对周边番茄产区产生危害。本研究对云南建水田间疑似ToBRFV感染的番茄果实样品进行电子显微镜观察及RT-PCR检测鉴定,并进一步对分离株进行全基因组测序分析,构建系统进化树分析其与其它ToBRFV分离株的进化关系。结果表明,从感病的番茄果实上发现杆状病毒粒体,大小约18 nm×300 nm,具有烟草花叶病毒属病毒的典型形态特征;用ToBRFV特异性检测引物进行RT-PCR扩增得到591 bp目的片段,经NCBI blast比对,与ToBRFV具有最高一致率(达99%以上),将该分离株命名为ToBRFV-2022-JS(GenBank登录号:OR593752)。测序发现ToBRFV-2022-JS基因组全长6386 nt,具有4个ORFs,分别编码126 kDa和183 kDa复制酶,30 kDa运动蛋白MP和17.5 kDa外壳蛋白CP。进化树分析显示该分离株与ToBRFV银川分离株(GenBank登录号:OR500698.1)的亲缘关系最近,核苷酸序列一致率为99.73%。上述结果表明云南建水番茄果实受到ToBRFV感染。该结果为ToBRFV在云南番茄产区的监测与防控提供了依据。
Tomato brown rugose fruit virus(ToBRFV)has been spreading rapidly in tomato-producing areas around the world in recent years,causing severe economic losses.Symptoms of suspected ToBRFV infection appeared on tomatoes in Jianshui,Yunnan Province,and caused harm to the surrounding tomato production areas.In this study,the tomato samples suspected of being infected with ToBRFV in the Yunnan Jianshui field were identified by electron microscopy and RT-PCR.RT-PCR amplified the whole genome sequence of the virus,and the phylogenetic tree was constructed to analyze the evolutionary relationship of the viruses.The results showed that the rod-shaped virions,about 18 nm×300 nm,with the typical structural characteristics of Tobamovirus,were found in the diseased tomato fruits.The target segment of 591 bp was amplified by RT-PCR using ToBRFV-specific detection primers.The Blast result in NCBI showed that this segment shared the highest identity(more than 99.00%)with the ToBRFV.We named this ToBRFV isolate as ToBRFV-2022-JS(GenBank accession number:OR593752).Sequencing result revealed that the complete sequence of ToBRFV-2022-JS was 6386 nt,with four ORFs encoding the 126 kDa and 183 kDa replicates,the 30 kDa movement protein(MP),and the 17.5 kDa capsid protein(CP),respectively.Phylogenetic tree analysis showed that this isolate was the closest relative to ToBRFV Yinchuan isolate(GenBank accession number:OR500698.1),with 99.73%nucleotide sequence identity.The above results indicated that tomato fruits in Jianshui,Yunnan were infected with ToBRFV.The results provide a basis for the monitoring,prevention,and control of ToBRFV in tomato production areas in Yunnan.
作者
肖雨晴
吕高莹
李树军
陈永对
苏晓霞
杨李源滨
周晓罡
余大朝
王田田
郑宽瑜
张仲凯
XIAO Yuqing;LÜGaoying;LI Shujun;CHEN Yongdui;SU Xiaoxia;YANG Liyuanbin;ZHOU Xiaogang;YU Dachao;WANG Tiantian;ZHENG Kuanyu;ZHANG Zhongkai(Biotechnology and Germplasm Resources Research Institute,Yunnan Academy of Agricultural Sciences,Yunnan Provincial Key Lab of Agricultural Biotechnology,Kunming 650205,China;Yunnan Shengu Technology Co.,Kunming 650220,China)
出处
《植物病理学报》
北大核心
2025年第2期194-202,共9页
Acta Phytopathologica Sinica
基金
云南省科技计划重大科技专项(202202AE090022)
神谷科技张仲凯专家工作站
云南种子种业联合实验室(202205AR070001)
云南省中青年学术和技术带头人后备人才项目(202305AC160026)。
