摘要
为了探究黄瓜花叶病毒(Cucumber mosaic virus, CMV)复制酶2a蛋白细胞核定位的生物学意义,以本氏烟UNE12蛋白(NbUNE12)为研究对象,通过双分子荧光互补实验及病毒诱导的基因沉默方法分析该蛋白与2a蛋白的相互作用情况及其在CMV侵染过程中的作用。结果表明:CMV 2a蛋白与NbUNE12蛋白的相互作用主要集中于细胞核中;下调表达NbUNE12增加CMV的2b缺陷型突变体(CMVΔ2b-GFP)在植物系统叶的病毒含量,表明NbUNE12蛋白能够与CMV 2a蛋白发生相互作用,并且负调控CMVΔ2b-GFP侵染本氏烟。该研究为CMV 2a蛋白在植物细胞核定位的生物学功能进一步研究奠定理论基础。
To investigate the biological significance of the nuclear localization of the cucumber mosaic virus(CMV)2a protein,the article,focusing on Nicotiana benthamiana UNE12 protein(NbUNE12),investigated the interaction between 2a and NbUNE12,as well as the functional role of Nb-UNE12 during CMV infection by conducting bimolecular fluorescence complementation experiments and employing the virus-induced gene silencing method.The findings indicate that the interaction between CMV 2a and NbUNE12 predominantly occured within the nucleus.Silencing NbUNE12 enhanced the accumulation of a 2b-deficient CMV mutant(CMVΔ2b-GFP)in the upper systemic leaves,suggesting that NbUNE12 interacted with CMV 2a and acted as a negative regulator of CMVΔ2b-GFP accumulation.This study lays a theoretical foundation for further study of the biological function of CMV2a protein localization in plant nucleus.
作者
姚佳怡
顾周杭
YAO Jiayi;GU Zhouhang(College of Life Sciences and Medicine,Zhejiang Sci-Tech University,Hangzhou 310018,China)
出处
《浙江理工大学学报(自然科学版)》
2025年第2期270-275,共6页
Journal of Zhejiang Sci-Tech University(Natural Sciences)
基金
浙江理工大学基本科研业务费项目(2021Q032)。
