摘要
目的:探讨四物汤对血虚小鼠红细胞膜Na+-K+-ATP酶的影响。方法:将200只雄性小鼠,随机分为10组,每组20只,分别制成:空白对照组;骨髓抑制性血虚模型对照组,药物治疗组,自然恢复组;溶血性血虚模型对照组,药物治疗组,自然恢复组;失血性血虚模型对照组,药物治疗组,自然恢复组。用酶学比色法测定红细胞膜上Na+-K+-ATP酶的活性。结果:失血性血虚和溶血性血虚四物汤治疗组红细胞膜Na+-K+-ATP酶活性升高与自然恢复组比较有显著性差异(P<0.01)。结论:四物汤能增高血虚时红细胞膜上Na+-K+-ATP酶的活性,能使红细胞恢复正常的形态和功能,对骨髓抑制性血虚四物汤疗效不明显。
ABSTRCT Objective:To explore the effect of Siwutang on the activity of Na + -K + -ATPase in the red cell membranes of the mice with blood deficiency.Method:200mice were randomly divided into blank control group(n=20),three kinds of blood deficiency model groups by myelosuppression,blood lose and hemolytic respectively.The control,treatment and natural recovery were set for every blood deficiency model group(n=20).The activities of Na + -K + -ATPase in the red cell membranes were tested by zymologic colorimetry.Results:The activity of Na + -K + -ATPase in the red cell membrane have decreased in all the blood deficiency mice.Siwutang could increase the activity of Na + -K + -ATPase in the membrane of RBC of the blood loss and hemolytic mice compared with natural recovery group(P<0.01).Conclusion:Siwutang could increase the activity of Na + -K + -ATPase in the membrane of RBC of the blood loss and hemolytic mice,and promote the function of RBC.But Siwutang could not increase the activity of Na + -K + -ATPase in the membrane of RBC of the myelosuppression mice.
出处
《海南医学院学报》
CAS
2003年第1期10-12,共3页
Journal of Hainan Medical University
关键词
血虚症
细胞膜
小鼠
药物作用
Blood deficiency sign
cell membrane
mouse
drug action
