摘要
以蓝莓灰葡萄孢菌(Botrytis cinerea Pers.)为供试菌,采用浸提、煎煮及水浴蒸馏3种方式对折耳根、大蒜、蒲公英、艾草及青蒿有效成分进行提取,通过平板抑菌实验测试其抑菌效果,并对各提取液对蓝莓离体果实发病率及品质指标的影响进行测定。结果表明,3种方式获得的提取液均能显著抑制灰葡萄孢菌菌丝生长;各提取液均可显著降低离体果实发病率(p<0.05),以大蒜、艾草效果最好(4%),折耳根、青蒿次之(12%),蒲公英效果最差(16%);另外,各提取液均可抑制蓝莓果实贮藏期间硬度的降低及失重率的增加,贮藏8 d后,可溶性固形物含量、pH值、维生素C含量、总花色苷等指标均高于对照组。表明上述5种提取液可有效抑制蓝莓灰霉病的发生并维持果实品质。
Blueberry gray mold pathogen Botrytis cinerea Pers.was used as test material,three extraction methods,namely soaking extraction,decoction,and water bath distillation,were employed to extract active ingredients from Houttuynia cordata,Allium sativum L.,dandelion,Artemisia princeps,and A.carvifolia.The antifungal effects of these extracts were evaluated through agar diffusion assays,and their impact on disease incidence and quality parameters of detached blueberry fruits were determined.The results indicated that extracts obtained through all three methods significantly inhibited the mycelium growth of B.cinerea.Moreover,all extracts significantly reduced the disease incidence of detached fruits compared to the control group(p<0.05).Among them,A.sativum L.and A.princeps extracts exhibited the best effect(4%of disease incidence),followed by H.cordata and A.carvifolia(12%),while dandelion extract showed the lowest effect(16%).Additionally,all extracts mitigated the decrease of hardness and increase of weight loss of blueberry fruits during storage.After 8 days of storage,the soluble solid content,pH value,vitamin C content,total flavonoid content,and other quality indicators of fruits treated with extracts were higher than those of the control group,which suggested that the five extracts effectively inhibited the occurrence of blueberry gray mold disease and maintained the fruit quality during storage.
作者
闵卓
杨羽茜
邹双强
张阳
江璐
MIN Zhuo;YANG Yuxi;ZOU Shuangqiang;ZHANG Yang;JIANG Lu(College of Brewing Engineering,Moutai Institute,Renhuai,Guizhou,564500,China)
出处
《中国南方果树》
北大核心
2025年第2期182-188,192,共8页
South China Fruits
基金
贵州省科技支撑计划(黔科合支撑[2020]1Y132,黔科合支撑[2021]一般115)
大学生创新创业训练计划项目(202114625003,S202214625011)资助。
关键词
蓝莓
植物提取物
灰霉菌
抑菌作用
采后品质
blueberry
plant extracts
Botrytis cinerea
inhibitory effect
post-harvest quality
