摘要
以灵芝加工后的菌柄为原料,研究其多糖、三萜的提取工艺及抗肿瘤、抗氧化活性。采用超声波-酶促辅助提取法优化加工废料多糖、三萜提取工艺;采用3-(4,5-二甲基-2-噻唑基)-2,5-二苯基四氮唑溴化物[3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide,MTT]法研究灵芝废弃物中三萜和多糖对两种癌细胞的抑制作用,并对多糖进行红外光谱表征;采用体外抗氧化模型对三萜和多糖进行活性研究。结果表明,松杉灵芝菌柄中多糖及三萜得率的最佳提取工艺条件为乙醇浓度40%、超声时间30 min、提取温度50℃、固液比1∶25(g/mL)、酶添加量500 U/g。在此条件下,多糖、三萜得率分别为(1.570±0.026)%、(1.635±0.020)%;多糖和三萜浓度与肝癌细胞(HepG2)的抑制率呈正相关,当多糖浓度达到1000μg/mL时,对HepG2和Caki-1两种肿瘤细胞的抑制率分别为(81.73±2.20)%、(71.39±2.73)%;当三萜浓度为500μg/mL时,对两种肿瘤细胞的抑制率分别为(64.33±1.16)%、(76.95±2.20)%;红外光谱结果显示该多糖为β-吡喃糖;体外抗氧化结果表明,该三萜、多糖均具有良好的抗氧化活性。
The extraction of polysaccharide and triterpenoids from the stipes(processing residues)of Gano⁃derma tsugae Murr was explored.Furthermore,the antitumor and antioxidant activities of the extracted polysaccharide and triterpenoids were determined.The ultrasound-assisted enzymatic method was used for extraction.The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide(MTT)method was employed to study the inhibitory effects of the extracted triterpenoids and polysaccharide on two cancer cell lines(HepG2 and Caki-1).The polysaccharide was characterized by infrared spectroscopy(IR).Furthermore,the antioxidant activities of triterpenoids and polysaccharide in vitro were determined.The results showed that the optimal extraction conditions were ethanol concentration of 40%,ultrasonic time of 30 min,ultrasonic temperature of 50℃,solid-liquid ratio of 1∶25(g/mL),enzyme dosage of 500 U/g.Under these conditions,the yields of polysaccharide and triterpenoids were(1.570±0.026)%and(1.635±0.020)%,respectively.The concentration of polysaccharide and triterpenoids was proportional to the inhibition rate on HepG2 cells.At the concentration of 1000μg/mL,polysaccharide showed the inhibition rates of(81.73±2.20)%and(71.39±2.73)%on HepG2 and Caki-1 cells,respectively.At the concentration of 500μg/mL,triterpenoids demonstrated the inhibition rates of(64.33±1.16)%and(76.95±2.20)%on HepG2 and Caki-1 cells,respectively.The IR showed that polysaccharide wasβ-pyranose.Both the extracted polysaccharide and triterpenoids showcased strong antioxidant activities in vitro.
作者
孙嘉泽
孙傲
吴桐
孟可逸
赵楠
柴军红
何婷婷
SUN Jiaze;SUN Ao;WU Tong;MENG Keyi;ZHAO Nan;CHAI Junhong;HE Tingting(Mudanjiang Normal University,Mudanjiang 157011,Heilongjiang,China)
出处
《食品研究与开发》
2025年第7期102-109,共8页
Food Research and Development
基金
黑龙江省教育厅项目资助(1452MSYYB006)
牡丹江师范学院科研平台项目(PT2020003)
牡丹江科技局攻关项目(G2018d2455)。
关键词
灵芝菌柄
多糖
三萜
抗肿瘤
抗氧化
提取工艺优化
stipe of Ganoderma tsugae
polysaccharide
triterpenoids
antitumor activity
antioxidant activity
extraction process optimization
