摘要
目的对乌毛蕨科狗脊属植物狗脊蕨Woodwardia japonica(L.f.)Smith和单芽狗脊蕨Woodwardia unigemmata(Makino)Nakai的药材进行区分和评价,为狗脊贯众药材的质量控制提供依据。方法采集不同产地的狗脊蕨贯众和单芽狗脊蕨贯众药材,建立定性定量测定方法对两种基源药材进行鉴别研究和质量比较。结果归纳整理了狗脊蕨贯众和单芽蕨狗脊贯众药材在性状、显微特征方面的差异,建立了专属性较强的TLC鉴别、HPLC鉴别和含量测定方法。二者性状及显微特征相似,但狗脊蕨叶柄残基横切面分体中柱2~4个,而单芽狗脊蕨为5~8个;狗脊蕨TLC色谱斑点和HPLC色谱峰均少于单芽狗脊蕨;狗脊蕨和单芽狗脊蕨中原儿茶醛与原儿茶酸的含量无明显差异。结论本研究可有效区分狗脊蕨贯众和单芽狗脊蕨贯众药材,为其质量评价和控制提供参考依据。
Objective To distinguish and evaluate Woodwardia japonica(L.f.)Smith and Woodwardia unigemmata(Makino)Nakai,and provide basis for the quality control of Woodwardiae rhizoma.Methods The medicinal materials of Woodwardia japonica(L.f.)Smith and Woodwardia unigemmata(Makino)Nakai from different origins were collected,and qualitative and quantitative determination methods were established to identify and compare the quality of two source medicinal materials.Results The differences between Woodwardia japonica(L.f.)Smith and Woodwardia unigemmata(Makino)Nakai in terms of charaters and microscopic charateristics were summarized and organized,and specialized TLC,HPLC identification and content determination methods were established.Although their macroscopic and microscopic characteristics were similar,the cross-section of leaf stalk remnants showed 2-4 vascular bundles in Woodwardia japonica(L.f.)Smith to 5-8 in Woodwardia unigemmata(Makino)Nakai.TLC analysis revealed fewer characteristic spots and HPLC chromatograms showed fewer peaks in Woodwardia japonica(L.f.)Smith than in Woodwardia unigemmata(Makino)Nakai.No significant differences were observed in the contents of protocatechualdehyde and protocatechuic acid between the two species.Conclusion This study can effectively distinguish Woodwardia japonica(L.f.)Smith and Woodwardia unigemmata(Makino)Nakai,and provide a reference for their quality evaluation and control.
作者
汪慧
范晓磊
蒋艳
WANG Hui;FAN Xiaolei;JIANG Yan(Wuhan Institute for Drug and Medical Device Control,Wuhan 430075,China)
出处
《药学前沿》
2025年第4期567-574,共8页
China Pharmacist
基金
湖北省科技重大专项重点研发计划项目(2020ACA007-01)。
关键词
狗脊蕨贯众
单芽狗脊蕨贯众
区分
黄酮
酚酸
薄层色谱
高效液相色谱
质量评价
Woodwardia japonica(L.f.)Smith
Woodwardia unigemmata(Makino)Nakai
Differentiation
Flavonoids
Phenolic acids
Thin layer chromatography
High performance liquid chromatography
Quality evaluation
