摘要
建立同时测定美白化妆品中10种植物原料指标性成分的超高效液相色谱-串联质谱(UPLC-MS/MS)方法。样品经80%(体积分数)甲醇水溶液超声提取后,采用Phenomenex Kinetex F5(3.0 mm×100 mm,2.6μm)色谱柱进行分离,以0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱。质谱采用电喷雾离子源(ESI),正、负离子同时扫描,多反应监测(MRM)模式进行检测。结果表明,10种指标性成分在各自的质量浓度范围内线性关系均良好(r>0.99),检出限(LOD)为0.07~1.93μg/kg,定量限(LOQ)为0.22~6.42μg/kg。在低、中、高3个质量浓度水平下,水基类、膏霜类、乳液类和面膜类基质样品中10种指标性成分的回收率为80.5%~122.5%,相对标准偏差(RSD)为1.3%~10.1%(n=6)。该方法准确灵敏,前处理简单,适用于多种基质类型的美白化妆品中多种植物原料指标性成分的定性鉴别及定量测定。
A method for simultaneous determination of 10 indicative components from plant materials in whitening cosmetics by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established.The sample was extracted by ultrasonic extraction using 80%methanol,then separated on a Phenomenex Kinetex F5 column(3.0 mm×100 mm,2.6μm)by gradient elution using 0.1%formic acid solution and acetonitrile as the mobile phases.The mass spectrometry was detected using electrospray ionization source(ESI),positive and negative ion simultaneous scanning and multiple reaction monitoring(MRM)mode.The results show that there are good linear relationships for 10 indicative components in their respective mass concentration rangers with the correlation coefficients(r)larger than 0.99.The limits of detection(LOD)are in the ranges of 0.07-1.93μg/kg and the limits of quantitation(LOQ)are in the ranges of 0.22-6.42μg/kg.The recoveries of toner,cream,emulsion and mask samples at three spiked levels for 10 indicative components are 80.5%-122.5%,with the relative standard deviations(RSD)of 1.3%-10.1%(n=6).With the advantages of accuracy,high sensitivity and simplicity,this method is suitable for qualitative identification and quantitative analysis of various indicative components from plant materials in various matrix types of whitening cosmetics.
作者
黄炜东
Weidong Huang(Quanzhou Institute for Food and Drug Control,Quanzhou,Fujian 362000,China)
出处
《日用化学工业(中英文)》
北大核心
2025年第4期531-538,共8页
China Surfactant Detergent & Cosmetics
