摘要
目的建立液液萃取–高效液相色谱法快速测定植物油中苯并[a]芘含量的方法。方法试样经乙腈振荡萃取,离心后取上清液过0.22μm滤膜,注入高效液相色谱仪进行测定,基质外标法定量。结果苯并[a]芘在质量浓度为0.5~20.0μg/kg线性范围内相关性良好,相关系数r^(2)≥0.9990;在0.5、2.0、10.0μg/kg质量浓度水平的加标回收率为87.0%~111.2%,相对标准偏差为2.2%~14.2%;方法检出限为0.2μg/kg,定量限为0.5μg/kg。结论本方法简单、快速、准确,适用于植物油中苯并[a]芘含量的测定。
Objective To establish a rapid method for the determination of benzo[a]pyrene in vegetable oil by liquidliquid extraction-high performance liquid chromatography.Methods The sample was extracted by acetonitrile oscillation,and after centrifugation,the supernatant was taken and passed through 0.22μm filter membrane,and injected into highperformance liquid chromatograph for measurement.Results were quantified using matrix external standard method.Results Benzo[a]pyrene had a good correlation in the linear range of 0.5-20.0μg/kg,and the correlation coefficient r^(2) was≥0.9990.The recoveries at 0.5,2.0 and 10.0μg/kg were 87.0%~111.2%,and the relative standard deviations were 2.2%~14.2%.The limits of detection and quantitation were 0.2μg/kg and 0.5μg/kg,respectively.Conclusion The method is simple,rapid and accurate,and is suitable for the determination of benzo[a]pyrene in vegetable oil.
作者
徐艺溶
利东玲
杨昆
梁宇
谭春翠
吴文静
秦富
XU Yi-Rong;LI Dong-Ling;YANG Kun;LIANG Yu;TAN Chun-Cui;WU Wen-Jing;QIN Fu(Technical Center of Nanning Customs District,Nanning 530201,China;School of Chemistry and Materials,Nanning Normal University,Nanning 530100,China)
出处
《实验室检测》
2024年第11期7-11,共5页
Laboratory Testing
基金
南宁市科学研究与技术开发计划项目(20212148)
关键词
植物油
苯并[A]芘
液液萃取
高效液相色谱法
vegetable oil
benzo[a]pyrene
liquid-liquid extraction
high performance liquid chromatography
