摘要
为制备溶菌酶肠溶微球,并对其进行质量评价和体外释放度考察,采用滴制法制备溶菌酶肠溶微球,以载药量、微球包封率为主要指标,正交设计优化溶菌酶肠溶微球的制备工艺。制得微球形态圆整,平均粒径(314.0±11.48)μm,包封率(91.13±1.89)%,载药量(11.66±0.76)%,微球在酸性介质中基本不释放,在肠溶介质中1 h释放低于30%,60 h释放达90%以上。该制备工艺稳定、操作简便,所制备微球包封率和载药量高,缓释效果好,可用于溶菌酶肠溶微球的制备。
To prepare lysozyme enteric microspheres,and evaluate its quality and release in vitro.The preparation of lysozyme enteric-coated microspheres by drop method was carried out,with the loading capacity and encapsulation efficiency as the main indicators.The preparation process was optimized by orthogonal design.The as-prepared microspheres had a good appearance with the particle size of(314.0±11.48)μm.The encapsulation efficiency and drug loading were(91.13±1.89)%and(11.66±0.76)%,respectively.The lysozyme enteric microspheres did not release in acidic medium,the accumulative release rate of lysozyme from microspheres was less than 30%in 1 h and more than 90%in 60 h in enteric medium.The optimized process is stable and feasible for preparing lysozyme enteric microspheres with high encapsulation efficiency and drug loading,and good sustained release effect.
作者
魏培
朱瑞娟
钱淼
杨文文
王娟弟
孙新堂
WEI Pei;ZHU Ruijuan;QIAN Miao;YANG Wenwen;WANG Juandi;SUN Xintang(Shandong Vocational Animal Science and Veterinary College,Weifang Shandong 261061,China;Gansu Institute for Drug Control,Lanzhou Gansu 730070,China;NMPA Key Laboratory for Quality Control of TCM,Lanzhou Gansu 730070,China;Weifang Key Laboratory of Animal Fermentation Technology,Weifang Shandong 261061,China)
出处
《现代畜牧科技》
2025年第2期42-44,共3页
Modern Animal Husbandry Science & Technology
基金
山东省农业重大技术协同推广项目(SDNYXTTG-2024-04)
中药材及饮片质量控制重点实验室项目(2023GSMPA-KL06,2024GSMPA-KL16)。
关键词
溶菌酶
微球
正交设计
质量评价
释放度
lysozyme
microsphere
orthogonal design
quality evaluation
release rate
