摘要
目的研究安息香Benzoinum化学成分以及体外抑制肿瘤增殖活性。方法采用MCI gel CHP-20P、正相硅胶、ODS和Sephadex LH-20柱色谱以及半制备型高效液相等色谱方法进行分离和纯化,通过红外、核磁共振、质谱等技术鉴定化合物的结构。CCK-8法评价分离得到的化合物体外抑制肿瘤增殖的活性。结果从安息香醋酸乙酯萃取部位中分离得到3个化合物,分别鉴定为1,3-二苯甲酸酯-2-(羟基(4-羟基-3-甲氧基苯基)甲基)丙烷(1)、5-乙酰基-7-甲氧基-2-(3,4-亚甲二氧基苯基)苯骈呋喃(2)和5-甲酰基-7-甲氧基-2-(3,4-亚甲二氧基苯基)苯骈呋喃(3)。化合物2和3对人肝癌Hep G2细胞增殖有抑制作用,半数抑制浓度(half maximal inhibitory concentration,IC_(50))值分别为41.2、52.6μmol/L。结论化合物1和2为新化合物,命名为白花树醇酯(1)和白花树醇酮(2);化合物3为新的天然产物;其中化合物2和3对人肝癌Hep G2细胞增殖表现出一定的细胞毒活性。
Objective To study the chemical constituents from Benzoinum and their antitumor activities in vitro.Methods The compounds were isolated and purified by MCI gel CHP-20P,silica gel,ODS,Sephadex LH-20 column chromatography and semi-preparative HPLC.Their structures were elucidated by spectroscopic analysis including IR,NMR and MS.CCK-8 assay was used to evaluate the inhibitory activity of the isolated compounds on tumor proliferation in vitro.Results A total of three compounds,1,3-dibenzoate-2-(hydroxy(4-hydroxy-3-methoxyphenyl)methyl)propane(1),5-acetyl-7-methoxy-2-(3,4-methylenedioxyphenyl)benzofuran(2),and 5-formyl-7-methoxy-2-(3,4-methylenedioxyphenyl)benzofuran(3),were isolated from the ethyl acetate extraction site of Benzoinum.Compounds 2 and 3 exhibited the inhibitory effects on HepG-2 cells with half maximal inhibitory concentration(IC_(50))values of 41.2,52.6μmol·L^(−1),respectively.Conclusion Compounds 1 and 2 are new compounds named styratonate(1)and styratonone(2),and compound 3 is a new natural product,among which compounds 2 and 3 exhibit certain cytotoxic activities on the proliferation of HepG2 cells.
作者
宋烨
黄菲
黄丽君
杨乐妍
王峰
方振峰
SONG Ye;HUANG Fei;HUANG Lijun;YANG Leyan;WANG Feng;FANG Zhenfeng(General pharmacy department of wuhan hospital of traditional Chinese medicine,Wuhan 430010,China;Department of Pharmacy,School of Medicine,Jianghan University,Wuhan 430056,China;Key Laboratory of Chinese Medicine Digital Quality Evaluation of National Administration of traditional Chinese medicine,Guangzhou 510006,China;Engineering&Technology Research Center for Chinese Materia Medica Quality of Guangdong Province,Guangzhou 510006,China)
出处
《中草药》
北大核心
2025年第3期760-764,共5页
Chinese Traditional and Herbal Drugs
基金
江汉大学科学专项基金资助项目(2023KYZX28)
广东省教育厅普通高校重点科研平台和项目(2022ZDZX2035)。
