摘要
目的检测精子相关抗原5(SPAG5)在肝内胆管癌(ICC)中的表达,分析其临床意义,明确及其对癌细胞增殖、迁移和侵袭的影响。方法选取2016年1月至2020年12月广东省东莞市水乡中心医院收治的6例配对新鲜和56例石蜡包埋ICC患者癌组织及癌旁正常组织作为研究对象,通过实时荧光定量聚合酶链式反应、蛋白免疫印迹法及免疫组织化学法分别检测SPAG5在新鲜与石蜡包埋ICC组织和ICC细胞株中的表达,分析其表达与ICC患者临床病理特征和预后相关性。使用5-乙炔基-2′-脱氧尿嘧啶核苷实验、克隆形成实验和Transwell小室实验检测过表达或敲降SPAG5后ICC细胞的增殖能力、克隆形成能力、迁移以及侵袭能力的改变。结果ICC癌组织中SPAG5 mRNA和蛋白表达均高于癌旁正常组织,差异有统计学意义(P<0.05)。与SPAG5低表达组比较,SPAG5高表达组有更短的无瘤生存期和总生存期(P<0.05)。过表达SPAG5可显著上调ICC细胞RBE、HCC C9810的增殖、迁移以及侵袭能力,敲低SPAG5表达可显著下调ICC细胞QBC939的增殖、迁移以及侵袭能力(P<0.05)。结论SPAG5在ICC中高表达预示ICC患者预后不良,并增强ICC的增殖、迁移及侵袭能力。
Objective To detect the expression of sperm-associated antigen 5(SPAG5)in intrahepatic cholangiocarcinoma(ICC),the clinical significance was analyzed,and its impact on cancer cell proliferation,migration,and invasion were clarified.Methods The 6 paired fresh and 56 paraffin embedded ICC patients'cancer tissues and adjacent normal tissues treated at Dongguan Waterfront Zone Central Hospital,Guangdong Province from January 2016 to December 2020 were collected and were as the research subjects,the expression of SPAG5 in fresh and paraffin embedded ICC tissues and ICC cell lines was detected by quantitative real-time polymerase chain reaction,western blot,and immunohistochemistry,respectively,the correlation between its expression and the clinical pathological characteristics and prognosis of ICC patients were analyzed.The 5-ethynyl-2'-deoxyuridine test,clone formation assay,and Transwell chamber assay were used to detect the changes in proliferation,clone formation,migration,and invasion ability of ICC cells after overexpression or knockdown of SPAG5.Results The expression of SPAG5 mRNA and protein in ICC cancer tissues were higher than those in adjacent normal tissues,and the differences were statistically significant(P<0.05).Patients with high expression of SPAG5 had shorter tumor free survival and overall survival than those with low expression of SPAG5(P<0.05).Overexpression of SPAG5 significantly upregulated the proliferation,migration,and invasion abilities of ICC cell RBE and HCC C9810,while knockdown of SPAG5 expression significantly downregulated the proliferation,migration,and invasion abilities of ICC cell QBC939(P<0.05).Conclusion The high expression of SPAG 5 in ICC predicts poor prognosis in ICC patients and enhances the proliferation,migration and invasion of ICC.
作者
罗莉彬
王娟
廖国平
简媛
陈泽康
陈渠发
LUO Libin;WANG Juan;LIAO Guoping;JIAN Yuan;CHEN Zekang;CHEN Qufa(Department of Gastroenterology,Dongguan Waterfront Zone Central Hospital,Guangdong Province,Dongguan523000,China;Department of Laboratory,Dongguan Waterfront Zone Central Hospital,Guangdong Province,Dongguan523000,China;Health Management Center,Dongguan Waterfront Zone Central Hospital,Guangdong Province,Dongguan523000,China)
出处
《中国当代医药》
2025年第6期31-35,共5页
China Modern Medicine
基金
广东省东莞市社会发展科技项目(20211800901542)。
关键词
肝内胆管癌
精子相关抗原5
增殖
迁移
侵袭
Intrahepatic cholangiocarcinoma
Sperm-associated antigen 5
Proliferation
Migration
Attack
