摘要
采用射频冷等离子体辅助提取技术从紫玉米芯中提取花青素,通过单因素和响应面优化实验,考察了放电功率、处理时间和工作压力对紫玉米芯花青素提取量的影响。利用超高效液相色谱仪-串联质谱仪(UPLC-MS)对紫玉米芯花青素的成分进行了分析和鉴定,使用秀丽隐杆线虫和H2O2分别对紫玉米芯花青素进行了抗氧化性能评价。结果表明,冷等离子体预处理的最佳条件为:放电功率127 W、处理时间75 s、工作压力150 Pa。在该条件下,紫玉米芯花青素提取量为(4.05±0.05)mg/g,较溶剂提取法和超声辅助提取法的提取量分别提高49.45%和28.98%。紫玉米芯花青素主要由天竺葵素-3-O-葡糖苷、矢车菊素-3-O-葡糖苷、矢车菊素和矮牵牛素-3-O-葡糖苷组成。与溶剂提取法和超声辅助提取法相比,冷等离子体辅助提取的花青素成分中矢车菊素-3-O-葡糖苷质量分数提高,天竺葵素-3-O-葡糖苷质量分数降低。紫玉米芯花青素清除1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS^(+))自由基的半抑制浓度(IC_(50))分别为0.2972、0.2770 g/L,接近同样条件下抗坏血酸对DPPH自由基和ABTS+自由基的清除能力。采用紫玉米芯花青素喂养的秀丽隐杆线虫,平均寿命延长29.26%;较H2O2处理组,其抗氧化能力提高了26.25%,体内活性氧水平降低了60.83%。
Radiofrequency cold plasma-assisted extraction technique was used to extract anthocyanins from purple maize cobs,with the effects of discharge power,pretreatment time and working pressure on anthocyanins extraction assessed by single factor and response surface analysis experiments.The composition of anthocyanins extracted from purple maize cobs was analyzed and identified by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS).And the antioxidant properties of the extracted anthocyanins were evaluated using Caenorhabditis elegans and H2O2,respectively.The results showed that the optimal conditions for cold plasma pretreatment were discharge power of 127 W,processing time of 75 s,and working pressure of 150 Pa.The yield of anthocyanins extracted reached(4.05±0.05)mg/g,49.45%and 28.98%higher than that of solvent extraction method and ultrasonic-assisted extraction method,respectively.Anthocyanins from purple maize cobs were mainly composed of pelargonidin-3-O-glucoside,cyanidin-3-O-glucoside,cyanidin and petunidin-3-O-glucoside.Compared with solvent extraction and ultrasonic-assisted extraction methods,cold plasma pretreatment led to increase in the mass fraction of cyanidin-3-O-glucoside,and reduction in pelargonidin-3-O-glucoside.The half maximal inhibitory concentration(IC_(50))of 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radicals and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid ammonium salt)(ABTS^(+))free radicals were 0.2972 and 0.2770 g/L,respectively,which were similar to those of ascorbic acid.The average life span of Caenorhabditis elegans fed with anthocyanins from purple maize cobs was extended by 29.26%,and the antioxidant capacity was increased by 26.25%.Compared with of those H_(2)O_(2)treatment group,the antioxidant capacity was increased by 26.25%,and the active oxygen level in vivo was decreased by 60.83%.
作者
徐小燕
朱国兵
张盟
龚磊
易霞
朱劼
XU Xiaoyan;ZHU Guobing;ZHANG Meng;GONG Lei;YI Xia;ZHU Jie(School of Pharmacy&School of Biological and Food Engineering,Changzhou University,Changzhou 213164,Jiangsu,China;School of Environmental Science and Engineering,Changzhou University,Changzhou 213164,Jiangsu,China;School of Petrochemical Engineering,Changzhou University,Changzhou 213164,Jiangsu,China;National-Local Joint Engineering Research Center of Biomass Refining and High-Quality Utilization,Changzhou 213164,Jiangsu,China)
出处
《精细化工》
北大核心
2025年第3期620-629,共10页
Fine Chemicals
基金
国家自然科学基金面上项目(22278040)。
