摘要
为研究防风(Saposhnikovia divaricata)地上部分的抗炎活性成分,该研究采用硅胶、ODS等柱色谱方法以及高效液相技术对70%乙醇提取物进行分离纯化,并结合理化性质及1H-NMR、13C-NMR、MS等波谱学数据对所分离化合物进行结构鉴定,采用脂多糖(LPS)诱导的RAW 264.7细胞模型测定化合物对一氧化氮(NO)的抑制活性。结果表明:(1)从防风地上部分中分离得到15个化合物,分别鉴定为姜糖脂A(1),(E)-2-己烯基-O-β-D-吡喃葡萄糖苷(2),(Z)-3-己烯基-O-β-D-吡喃葡萄糖苷(3),正己烷基-O-β-D-吡喃葡萄糖苷(4),sachalinoside B(5),5β,6α-dihydroxy-3β-(β-D-glucopyranosyloxy)-7-megastigmen-9-one(6),phenethyl-β-D-glucopyranoside(7),没食子酸乙酯(8),香草酸(9),蚱蜢酮(10),2-乙氧基-2-对羟基苯基乙醇(11),2-methoxy-2-(4′-hydroxyphenyl)ethanol(12),1,2,3,4,6-penta-O-gally-β-D-glucopyranose(13),(-)-当归棱子芹醇-2-O-β-D-呋喃芹菜糖基-(1→6)-β-D-吡喃葡萄糖苷(14),(9Z,12Z)-N-(2-hydroxyethyl)octadeca-9,12-dienamide(15)。其中,化合物1-5、7-10首次在伞形科植物中分离得到,化合物11-15首次从防风属植物中发现。(2)对化合物1-15进行了体外抗炎活性实验,结果表明化合物1、3、4、7、9、12、14对LPS诱导的RAW 264.7细胞NO释放具有不同程度的抑制作用。
In order to investigate chemical components and anti-inflammatory activities from the aerial parts of Saposhnikovia divaricata.Silica gel,ODS and HPLC,etc.were utilized for the separation and purification of the 70%ethanol extract.The structures of the isolated compounds were characterized by integrating physicochemical properties and spectroscopic data including 1 H-NMR,13 C-NMR,and MS.Their inhibitory activity of the compounds on nitric oxide(NO)was determined by lipopolysaccharide(LPS)-induced RAW 264.7 cell model.The results were as follow:(1)Fifteen compounds were isolated from the aerial parts of Saposhnikovia divaricata.They were identified as gingerglycolipid A(1),(E)-2-hexenyl-O-β-D-glucopyranoside(2),(Z)-3-hexenyl-O-β-D-glucopyranoside(3),hexyl-O-β-D-glucopyranoside(4),sachalinoside B(5),5β,6α-dihydroxy-3β-(β-D-glucopyranosyloxy)-7-megastigmen-9-one(6),phenethyl-β-D-glucopyranoside(7),ethylgallate(8),vanillic acid(9),grasshopper ketone(10),2-ethoxy-2-(4-hydroxyphenyl)-ethanol(11),2-methoxy-2-(4′-hydroxyphenyl)ethanol(12),1,2,3,4,6-penta-O-gally-β-D-glucopyranose(13),(-)-angelica angellinol-2-O-β-D-furan celery glycosyl-(1→6)-β-D-glucopyranoside(14),(9Z,12Z)-N-(2-hydroxyethyl)octadeca-9,12-dienamide(15).Among them,compounds 1-5 and 7-10 were isolated from plants of the Umbelliferae for the first time,and compounds 11-15 were discovered from plants of the Saposhnikovia genus for the first time.(2)In vitro anti-inflammatory activity experiments were conducted on compounds 1-15.The results showed that compounds 1,3,4,7,9,12 and 14 could inhibit the release of NO from RAW 264.7 cells induced by LPS.
作者
刘振强
周淑鑫
周露瑶
林宇轩
刘爽
张倩
孙艳
陈庆山
张丽莉
匡海学
刘艳
杨炳友
LIU Zhenqiang;ZHOU Shuxin;ZHOU Luyao;LIN Yuxuan;LIU Shuang;ZHANG Qian;SUN Yan;CHEN Qingshan;ZHANG Lili;KUANG Haixue;LIU Yan;YANG Bingyou(Key Laboratory of Basic and Application Research of Northern Medicine of Ministry of Education,College of Pharmacy Heilongjiang University of Chinese Medicine,Harbin 150040,China;College of Agriculture,Northeast Agricultural University,Harbin 150030,China)
出处
《广西植物》
北大核心
2025年第2期207-216,共10页
Guihaia
基金
国家自然科学基金区域联合创新基金重点项目(U22A20370)
黑龙江省重点研发计划项目(GA21D008)
黑龙江省中医药管理局中医药科研课题项目(ZHY2024-001)
黑龙江省“双一流”学科协同创新成果建设项目(LJGXCG2022-096)。
关键词
防风
地上部分
化学成分
提取分离
结构鉴定
抗炎活性
Saposhnikovia divaricata
aerial part
chemical components
extraction and separation
structural identification
anti-inflammatory activity
