摘要
使用转录组分析方法,模拟4个含镉海洋环境(0、0.01、1.0、10.0 mg/L),从基因表达水平探究史氏背尖贝(Nipponacmea schrenckii)响应镉胁迫的行为模式,比较不同镉浓度之间的基因表达量变化,筛选对镉离子浓度变化敏感的差异基因。结果表明:转录组数据共组装出261922个unigene,其中32.30%的unigene被注释到7个常用数据库中;基于NR注释发现史氏背尖贝与霸王莲花青螺(Lottia gigantea)在基因表达方面具有较高同源性;挖掘出2021个响应镉胁迫的差异基因,其中随着镉浓度的增加有238个基因表达水平持续上调。CTSL、GST、GSTO 1基因对镉浓度变化相对敏感,可以作为指示海洋镉污染的潜在生物标志物基因。
The RNA-Seq analysis method is applied to investigate the behavioral patterns of Nipponacmea schrenckii in response to Cd 2+stress,and to compare the changes in gene expression levels at different Cd 2+concentrations(0,0.01,1.0,and 10.0 mg/L).A total of 261922 unigenes are assembled,and 32.30%of them are annotated in seven commonly used databases.Based on NR annotation,N.schrenckii shares a high degree of homology with Lottia gigantea.A total of 2021 differentially expressed genes(DEGs)are identified in response to Cd 2+stress,and 238 of these genes have higher expression levels with the increasing the Cd 2+concentration.The CTSL,GST,and GSTO 1 genes are found to be relatively sensitive to changes in Cd 2+concentration and could be potential biomarker genes for marine Cd 2+pollution.
作者
薛浩
王成红
王丽君
刘秀梅
邢志凯
王爽
王绪敏
曲江勇
XUE Hao;WANG Chenghong;WANG Lijun;LIU Xiumei;XING Zhikai;WANG Shuang;WANG Xumin;QU Jiangyong(School of Life Sciences,Yantai University,Yantai 264005,China)
出处
《烟台大学学报(自然科学与工程版)》
2025年第1期17-29,共13页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
山东省重点研发计划(重大科技创新工程)(2021CXGC011306)。
