摘要
研究了低浓度(1、3、6、9μg/L)Cd2+长期(28 d)作用对泥蚶(Tegillarca granosa)内脏团和鳃两种组织的总超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)及丙二醛(MDA)含量的影响。结果发现:与对照组相比,四个浓度组Cd2+对泥蚶鳃和内脏团两种组织的抗氧化酶体系均产生显著影响。除最高浓度组外,其余浓度组鳃和内脏团组织SOD活性前21 d均被诱导,第28 d时,所有组内脏团组织SOD活性被显著抑制,最高浓度组两种组织SOD活性第7d时被抑制,此后被诱导;除9μg/L浓度组外,其余浓度组鳃组织CAT活性与Cd2+浓度呈剂量效应关系,前14 d时被抑制,后14 d时被诱导;内脏团组织GSH-PX活性变化规律不明显;内脏团组织中产生的MDA含量显著高于鳃组织,所有组鳃组织中MDA含量均在第7 d时达到最高值,而内脏团组织中MDA含量仍在增加。研究发现,泥蚶鳃组织中CAT活性可以作为低浓度Cd2+污染的生物标志物;现今的国家渔业水质标准所规定的Cd2+浓度(≤0.005 mg/L)在泥蚶等双壳贝类上的适用性还有待探讨。
We investigated the effect of long-term stress of low concentrations of Cd^2＋ on antioxidant enzyme activities（ superoxide dismntase（ SOD）, catalase（ CAT）, glotathione peroxidase（GSH-PX） ） and the content of MDA in the gills and visceral mass of Bloody clam. The results showed that：compared to the control group, the bloody clam of all concentration groups had been significantly effected by Cd^2＋ . The SOD of all concentration groups were induced in the first 21 days except the highest concentration group, and the SOD in visceral mass of all concentration groups were significantly inhibited after 28 days. The SOD of the highest concentration group was inhibited after 7 days,and then was induced. The CAT in gills of 1,3 and 6 μg/L groups had a good dose-effect relationship with the concentration of Cd^2＋ ,they were inhibited during the first 14 days and then induced in the following 14 days. The variation of GSH-PX was not obvious. The content of MDA in visceral mass was significantly higher than that in gills, At 7th day, the content of MDA in gills of all concentration group had came to the highest value,while in visceral mass , it was still increasing. The study suggested that the CAT activity in gills of Bloody clam can be used as a biomarker of low-concentration Cd^2＋ pollution ; and the applicability of National Fisheries water quality standards under the Cd^2＋ concentration（ ≤0. 005 mg/L） in shells as Bloody clam remains to be explored.
出处
《海洋环境科学》
CAS
CSCD
北大核心
2013年第5期741-745,共5页
Marine Environmental Science
基金
国家贝类产业技术体系(CARS-48)
宁波市科技创新团队项目(2011B82017)
宁波市自然科学基金(2011A610016)
宁波市科技局重点科技攻关计划项目(2011C11004)
宁波市国际合作项目(2010D10017)
