摘要
本研究旨在探讨外源水杨酸(Salicylic Acid,SA)对三七根系代谢的影响,并研究其显著变化的差异代谢物对三七主要根腐病菌生长的影响,以期为三七根腐病的生态防控提供新的思路。通过在连作条件下的三七叶部喷施不同浓度的SA,筛选减轻三七根腐病的最佳SA浓度。在此基础上,利用气相色谱-质谱联用仪(GC-MS)分析了最佳浓度下三七须根代谢物的变化,并进一步研究了显著变化的代谢物对三七根腐病原菌生长的影响。研究结果表明,与对照相比,外源施用280.00μg/mL SA能够显著提高连作土中三七的存苗率,并显著促进根长和生物量的增长。代谢组学分析显示,三七须根代谢物主要包括有机酸、氨基酸及其衍生物、糖类及其衍生物等九类物质。外源施用SA后,三七根系的糖代谢和脂肪酸代谢发生显著变化,其中D-(+)-海藻糖和亚油酸的含量显著下降。进一步研究表明,这两种显著下调的代谢物在一定浓度范围内均能显著促进主要根腐病菌的生长。该研究结果为三七根腐病的生态防控提供了理论基础和技术支撑。
The study investigated the effects of exogenous salicylic acid(SA)on root metabolism of Panax notoginseng,explored how differentially expressed metabolites influenced the growth of the main root rot pathogens of P.notoginseng,aiming to provide novel ideas for the ecological prevention and control of P.notoginseng root rot.Different concentrations of SA were sprayed on the leaves of P.notoginseng under continuous cropping conditions to screen the optimal SA concentration for reducing root rot disease.Gas chromatography-mass spectrometry(GC-MS)was then used to analyze the changes in metabolites in the fibrous roots of P.notoginseng at the optimal SA concentration,and the effects of significantly changed metabolites on the growth of P.notoginseng root rot pathogens were further studied.The results showed that compared with the control,exogenous application of 280.00μg/mL SA could significantly increase the seedling survival rate of P.notoginseng in continuous cropping soil,and significantly promote the growth of root length and biomass.Metabolomics analysis revealed that the metabolites in the fibrous roots of P.notoginseng mainly included nine types of substances,such as organic acids,amino acids and their derivatives,sugars and their derivatives.After exogenous application of SA,the sugar metabolism and fatty acid metabolism of P.notoginseng roots changed significantly,and the contents of D-(+)-trehalose and linoleic acid decreased significantly.Further studies showed that these two significantly down-regulated metabolites could significantly promote the growth of the main root rot fungi within a certain concentration range.The results of this study provide a theoretical basis and technical support for the ecological prevention and control of P.notoginseng root rot.
作者
陈胜倩
王正平
施雨宇
尚昭仪
朱书生
杨敏
CHEN Shengqian;WANG Zhengping;SHI Yuyu;SHANG Zhaoyi;ZHU Shusheng;YANG Min(Key Laboratory of Agrobiodiversity and Pest Management,Ministry of Education/State Key Laboratory for Conservation and Utilization Bio-resources in Yunna/College of Plant Protection,Yunnan Agricultural University,Kunming 650201,China)
出处
《中国生物防治学报》
北大核心
2025年第1期177-185,共9页
Chinese Journal of Biological Control
基金
国家自然科学基金联合基金(U23A20202)
云南省农业联合重点项目(202101BD070001-003)
昆明市科技揭榜制项目(2021JH002)。
关键词
水杨酸
海藻糖
亚油酸
三七根腐病
salicylic acid
trehalose
linoleic acid
Panax notoginseng root rot
