摘要
【目的】从睡莲基因组中鉴定NAC成员,并对其进化历程以及在不同组织和ABA胁迫下的表达模式进行分析,为睡莲NAC家族的分子进化提供理论基础,同时为睡莲NAC成员调控花器官形态建成和响应逆境胁迫提供候选基因。【方法】利用全基因组方法分析睡莲NAC家族成员的保守基序、基因结构、系统进化、染色体定位、基因复制事件、启动子顺式调控元件,并使用转录组和RT-qPCR对睡莲NAC家族成员在不同组织和ABA胁迫下的表达模式进行分析。【结果】从睡莲基因组中筛选出64个NAC成员,可分为15个亚家族。NcNAC成员不均匀地分布在睡莲14条染色体上,大部分成员定位于1、2、9和11号染色体上。睡莲NAC家族的片段复制事件与睡莲NAC家族的扩增紧密联系。NcNAC11、NcNAC43、NcNAC36和NcNAC52在花器官间的表达量较高,推断这些基因可能参与睡莲花器官的形态建成。NcNAC成员启动子区含有大量与胁迫响应相关的顺式调控元件,其中启动子区含有ABA响应元件最多的NcNAC07、NcNAC08、NcNAC34和NcNAC43能够响应ABA胁迫,表明NcNAC07、NcNAC08、NcNAC34和NcNAC43可能与睡莲的抗逆胁迫有关。【结论】在睡莲中有64个NcNAC成员,可以将其分为15个亚家族,NcNAC11、NcNAC43、NcNAC36和NcNAC52在花器官中的表达量较高,NcNAC07、NcNAC08、NcNAC34和NcNAC43能够响应ABA胁迫。
【Objective】This study aims to identify the NAC members in waterlily(Nymphaea colorata),and to investigate their evolutionary history and expressions under different tissues and ABA treatment.The current study not only could provide a key theoretical basis on the molecular evolution of the waterlily NAC family,but also could obtain some important candidate genes about regulating floral organ morphogenesis and stress response in waterlily.【Method】The whole-genome methods were applied to analyze the conserved motifs,gene structures,phylogenetic evolutions,chromosomal locations,gene duplication events,and cis-regulatory elements in the promoter regions of the waterlily NAC family.The expression levels of the waterlily NAC members under different tissues and ABA treatment were performed through the transcriptome and RT-qPCR.【Result】In this study,64 NAC members screened from waterlily were divided into 15 subfamilies.The NcNAC members were unevenly distributed on the waterlily’s 14 chromosomes,with most members located on chromosomes 1,2,9,and 11.Segmental duplication events were prevalent in the expansion of the NcNAC members.Meanwhile,NcNAC11,NcNAC43,NcNAC36 and NcNAC52 were highly expressed in floral organs.Thus,NcNAC11,NcNAC43,NcNAC36 and NcNAC52 may regulate the flower growth and development in waterlily.In addition,numerous stress-responsive regulatory elements were found in the promoter regions of the NcNAC members.NcNAC07,NcNAC08,NcNAC34,and NcNAC43 had the most ABA responsive elements in their promoter regions,and these NcNAC members responded to ABA stress,indicating that NcNAC07,NcNAC08,NcNAC34,and NcNAC43 might be related to stress resistance.【Conclusion】There are 64 NcNAC members in waterlily,and these NcNACs could be classified into 15 subfamilies.NcNAC11,NcNAC43,NcNAC36 and NcNAC52 have the highest expression in the floral-related tissues,and NcNAC07,NcNAC08,NcNAC34,and NcNAC43 may respond to ABA stress.
作者
钱政毅
吴绍芳
曹舒怡
宋雅欣
潘鑫峰
李兆伟
范凯
QIAN Zheng-yi;WU Shao-fang;CAO Shu-yi;SONG Ya-xin;PAN Xin-feng;LI Zhao-wei;FAN Kai(Key Laboratory of Genetics,Breeding and Multiple Utilization of Crops,Ministry of Education,College of Agriculture,College of Juncao Science and Ecology,Fujian Agriculture and Forestry University,Fuzhou 350002)
出处
《生物技术通报》
北大核心
2025年第2期234-247,共14页
Biotechnology Bulletin
基金
福建省自然科学基金项目(2021J01073)
福建农林大学科技创新专项基金(KFb22006XA)
福建农林大学访学研修项目(KFXZ23004)。
关键词
睡莲
NAC家族
转录因子
分子进化
表达分析
Nymphaea colorata
NAC family
transcription factor
molecular evolution
expression analysis
