摘要
【目的】水稻的粒长、粒宽和千粒质量是重要的品质与产量性状,特殊粒形材料的研究可为水稻品种粒形的遗传改良提供重要的基因资源。【方法】以常规粳稻品种日本晴和籼稻品种黄华占为参考,对2个水稻特大粒材料lg1和lg2基因组中9个粒形主效基因(qGL3、GS3、qLGY3、GS9、GW2、GW5、GW8、TGW3、TGW6)的功能变异位点进行了分析。【结果】与日本晴相比,lg1和lg2中粒长基因GS3和粒宽基因GW2分别在编码区第165位和第382位发生单碱基替换,其中突变分别导致了GS3翻译提前终止和GW2功能减弱,而GW8在5'非翻译区存在10 bp的插入,导致其功能减弱;此外,lg2中qLGY3发生了31 bp的缺失,导致其编码区发生了移码,其他粒形基因没有检测到功能发生明显变化的变异。与黄华占相比,在lg1和lg2中除检测到GW2和qLGY3的功能变异位点外,还在2个材料的GW5编码区上游检测到了950 bp的片段缺失。【结论】lg1基因组聚合了GS3、GW2、GW5、GW8的功能缺失或减弱的变异位点,lg2基因组中聚合了GS3、GW2、GW5、GW8、qLGY3的功能缺失或减弱的变异位点,研究结果为粒形基因的遗传互作及特大粒材料lg1和lg2粒形形成的机制解析提供依据。
[Objective]The grain length,grain width,and 1000-grain weight are the important traits determining rice quality and yield.The study of special grain shape materials can provide important genetic resources for the genetic improvement of rice grain shape.[Method]In this study,the functional variation sites of nine major genes(qGL3,GS3,qLGY3,GS9,GW2,GW5,GW8,TGW3,TGW6)in the genomes of two rice super-large grain materials lg1 and lg2 were analyzed with the conventional japonica rice variety Nipponbare and indica rice variety Huanghuazhan as reference.[Result]The results suggested that compared with Nipponbare,there was a single nucleotide substitution at positions 165 bp and 382 bp in coding regions of GS3 and GW2 in lg1 and lg2.The mutations resulted in the premature termination of GS3 translation and a weakened function of GW2.A 10 bp insertion in the 5'untranslated region of GW8 were in lg1 and lg2,which weakened its function.Furthermore,a frameshift mutation occurred in the coding region of lg2 due to a 31 bp deletion of qLGY3.No significant functional variation was detected in other grain shape genes.In addition to the functional variation sites of GW2 and qLGY3 in lg1 and lg2,a 950 bp fragment deletion was detected in the upstream of GW5 coding region of the two materials.[Conclusion]The lg1 genome aggregated the loss-of-function or weakened mutation sites of GS3,GW2,GW5,and GW8,and the lg2 genome aggregated the loss-of-function or weakened mutation sites of GS3,GW2,GW5,GW8,and qLGY3.Our results lay the foundation for the genetic interaction of grain shape related genes and the mechanism analysis of grain shape formation in lg1 and lg2.
作者
汪欲鹏
杜一帆
陈慧珍
王智权
李昕雨
邓梓阳
吴自明
WANG Yupeng;DU Yifan;CHEN Huizhen;WANG Zhiquan;LI Xinyu;DENG Ziyang;WU Ziming(Jiangxi Agricultural University/Key Laboratory of Crop Physiology,Ecology and Genetic Breeding,Ministry of Education,Nanchang 330045,China;Pingxiang Municipal Center of Agricultural Science,Pingxiang,Jiangxi 337099,China;Institute of Rice,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China)
出处
《江西农业大学学报》
北大核心
2025年第1期1-9,共9页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家重点研发计划项目(2023YFD2301301)
江西省重点研发计划项目(20232BBF60001)
江西省“稻稻油”农业科技项目(JXNK202302-02)。
关键词
水稻
粒形
千粒质量
大粒材料
粒形基因
遗传变异
rice
grain shape
1000-grain weight
big grain germplasm
grain shape genes
genetic variation
