摘要
利用硅胶、Sephadex LH-20、MCI Gel和ODS等柱色谱,结合半制备液相等分离方法,从羊角棉90%乙醇提取物中分离纯化得到9个化合物。运用现代波谱学方法(1D和2D NMR、UV、IR、MS等)对所分离得到的化合物进行结构鉴定,分别鉴定为mairoside A(1)、3′,6-二-O-芥子酰基蔗糖(2)、肉豆蔻醚酸(3)、肉豆蔻醚酸甲酯(4)、肉豆蔻醚酸乙酯(5)、3,4,5-三甲氧基桂皮酸(6)、3,4,5-三甲氧基苯甲酸(7)、维诺林(8)、山柰酚-3-O-芸香糖苷(9),其中化合物1为新糖苷类化合物,化合物4和5为新天然产物,且化合物4的核磁数据为首次报道。
Nine compounds were isolated and purified from 90%ethanol extract of Alstonia mairei Lévl by using various chromatographic methods,including silica gel,SephadexLH-20,MCI Gel and ODS column chromatography,combined with semi-preparative liquid phase separation methods.Modern spectroscopic methods(ID and 2D NMR,UV,IR,MS,etc.)were used to identify the structures of the isolated compounds.They were identified as mairoside A(1),3',6-di-O-sinaloylsucrose(2),myristic acid(3),methyl myristate(4),ethyl myristate(5),3,4,5-trimethoxycinnamic acid(6),3,4,5-trimethoxybenzoic acid(7),vinoline(8),kaempferol-3-O-rutinoside(9),among which compound 1 is a new glycoside,compounds 4 and 5 are new natural products,and the nuclear magnetic data of compound 4 were reported for the first time.
作者
王立柯
李冰燕
赵珍珠
王彦志
李晓坤
冯卫生
司盈盈
WANG Li-ke;LI Bing-yan;ZHAO Zhen-zhu;WANG Yan-zhi;LI Xiao-kun;FENG Wei-sheng;SI Ying-ying(School of Pharmacy,Henan University of Traditional Chinese Medicine,Zhengzhou 450046,China;Engineering and Technology Center for Chinese Medicine Development of Henan Province,Zhengzhou 450046,China)
出处
《药学学报》
北大核心
2025年第1期191-195,共5页
Acta Pharmaceutica Sinica
基金
河南省科技攻关项目(232102310383)
河南省高校科技创新团队支持计划资助(24IRTSTHN039)。
关键词
鸡骨常山属
羊角棉
化学成分
糖苷
分离鉴定
Alstonia
Alstonia mairei Levl.
chemical composition
glycoside
separation and structural characterization
