摘要
柚皮苷是一种具有广泛用途的二氢黄酮类化合物,为建立基于碱提酸沉柚皮苷提取工艺的质量标准提供物质基础。通过反复重结晶,正向柱层析,制备液相及半制备液相等方法分离纯化,经NMR,圆二色谱等手段鉴定结构。从微量杂质中分离鉴定12个杂质,分别为佛手酚(1)、7-Hydroxy-1-benzopiran-2-one(2)、Marmin(3)、Protocatechuic acid(4)、8-(6,7-dihydroxy-3,7-dimethyloct-1-en-3-yl)-5-hydroxy-6,7-furocoumarin(5)、Obacunone(6)、柚皮素(7)、7,4′-Dihydroxyflavone(8)、Rhoifolin(9)、Vicenin(10)、Neoeriocitrin(11)和2 S-Neoeriocitrin(12)。化合物5为首次从柚的果实中分离得到,为建立碱提酸沉工艺提取柚皮苷的质量标准建立提供物质基础。
Naringin is a kind of flavonoid with multiform usages.The impurities were isolated and identified from the alkali extraction and acid precipitation process of naringin.Repeated recrystallization,column chromatography,preparative and semipreparative HPLC was employed for isolation and purification.Then the purified impurities were identified by NMR and CD spectra.The twelve impurities were obtained and determined as Bergaptol(1),7-Hydroxy-1-benzopiran-2-one(2),Marmin(3),Protocatechuic acid(4),8-(6,7-dihydroxy-3,7-dimethyloct-1-en-3-yl)-5-hydroxy-6,7-furocoumarin(5),Obacunone(6),Naringin(7),7,4′-Dihydroxyflavone(8),Rhoifolin(9),Vicenin(10),Neoeriocitrin(11)and 2 S-Neoeriocitrin(12).The above twelve impurities will help to establish quality control standards of naringin based on alkali extraction and acid precipitation process.
作者
张丽
唐官美
韦媛
卢振鸿
韩方方
张雪晴
郭志勇
ZHANG Li;TANG Guan-mei;WEI Yuan;LU Zhen-hong;HAN Fang-fang;ZHANG Xue-qing;GUO Zhi-yong(College of Biological and Pharmaceutical Sciences,China Three Gorges University,Yichang 443002,China;HuBei Key Laboratory of Natural Products Research and Development,China Three Gorges University,Yichang 443002,China)
出处
《化学试剂》
CAS
2025年第1期61-66,共6页
Chemical Reagents
关键词
柚皮苷
碱提酸沉提取工艺
香豆素
黄酮类化合物
杂质
naringin
alkali extraction and acid precipitation process
coumarins
flavonoids
impurities
