摘要
为有效鉴别鲍的种类,探讨其来源,通过高通量测序获得福建养殖皱纹盘鲍线粒体基因组全序列,分析比较其序列与GenBank中收录的3个皱纹盘鲍线粒体基因组全序列或近全序列。试验结果显示,皱纹盘鲍线粒体基因组全长17037 bp,具有37个编码基因,包括13个蛋白质编码基因、22个tRNA基因和2个rRNA基因。除控制区存在碱基差异外,当前4个皱纹盘鲍线粒体全序列基本一致。基于鲍属线粒体基因组全序列构建系统发育树,4个皱纹盘鲍聚为一支,再与红鲍、黑鲍及黑足鲍聚为一支,其中福建养殖皱纹盘鲍连江群体与韩国巨济群体关系较近。笔者分析了不同皱纹盘鲍群体间的线粒体碱基组成和鲍属贝类系统进化关系,试验结果丰富了皱纹盘鲍遗传信息库,可为东南沿海鲍种的鉴定和良种选育提供参考。
In order to effectively identify abalone species and explore the sources,the complete mitochondrial genome of disk abalone Haliotis discus hannai cultured in Fujian province was sequenced by high-throughput sequencing technologies.The sequences obtained were preliminarily analyzed and the whole genome was compared with three complete or nearly-complete mitochondrial genome sequence of the disk abalone deposited in GenBank.The results showed that the disk abalone had mitogenome of 17037 bp in total length with 37 genes identified including 13 protein coded,22 tRNA and 2 rRNA genes.Except for the nucleotide bases differences in the control region,basically the same complete or nearly-complete mitochondrial sequences were observed in the four populations of the disk abalone.A phylogenetic tree constructed based on reported complete mitochondrial genome sequences of species in Haliotis genus revealed that all disk abalone were clustered with each other first and then clustered with H.rufescens,H.cracherodii and H.iris.The disk abalone from Lianjiang,China,had a close relationship with those from Geoje,Republic of Korea.In brief,sequencing and analysis of the entire mitochondrial genome of the abalone cultivated in Fujian province enriched the genetic information database of this species.The finding provides reference for the identification and breeding of high-quality abalone varieties in the southeastern coastal waters of China.
作者
王伟
WANG Wei(Fuzhou Ocean and Fisheries Technology Center,Fuzhou 350007,China;Marine and Fisheries Technology Research Center,Institute of Oceanography of Fuzhou,Fuzhou 350108,China)
出处
《水产科学》
北大核心
2025年第1期100-107,共8页
Fisheries Science
基金
福建省科技厅项目(2021S0010)
福州市科技局项目(2023-N-012)
福州海洋研究院项目(2021F12)。
关键词
皱纹盘鲍
线粒体基因组
系统发育
高通量测序
Haliotis discus hannai
mitochondrial genome
phylogeny
high-throughput sequencing
