摘要
茶小绿叶蝉是茶园重要虫害之一,严重影响茶叶的产量和品质。前期研究表明茶树TIFY转录因子家族在茶小绿叶蝉侵害过程中发挥重要作用。本研究对6个CsTIFY基因进行SSR位点检索并设计了132条引物,通过毛细管电泳筛选了5条多态性明显、条带清晰的SSR条带。结合24份茶树种质资源的茶小绿叶蝉抗性表型,发现SSR分子标记的分型成功率从58.3%(CsTIFY2-7)到83.3%(CsTIFY4-33)。本研究结果可以为茶树抗小绿叶蝉的早期鉴定及分子遗传育种提供理论基础。
The tea green leafhopper,an important pest in tea plantations,exerts severe negative impacts on both the yield and quality of tea.Previous research has demonstrated the crucial role played by the gene TIFY transcription factor family in conferring resistance against this insect in tea plants.In this study,we conducted SSR locus screening for six CsTIFY genes and subsequently designed 132 primers.Through capillary electrophoresis,we obtained five SSR molecular markers with distinct and polymorphic bands.Furthermore,our findings revealed that the genotyping success rate of SSR molecular markers varied from 58.3%(CsTIFY2-7)to 83.3%(CsTIFY4-33)when combined with resistance phenotypes against tea green leafhopper in 24 tea germplasm resources.These results serve as a robust foundation for early identification of resistance to tea green leafhopper and molecular genetic breeding in tea plants.
作者
李达
汪瑛琦
卢秦华
任苧
李清声
LI Da;WANG Yingqi;LU Qinhua;REN Ning;LI Qingsheng(Institute of Sericulture and Tea,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,Zhejiang,China;College of Tea Science and Tea Culture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)
出处
《茶叶》
2024年第4期227-231,共5页
Journal of Tea
基金
浙江省“十四五”农业(茶树)新品种选育重大科技专项(2021C02067-7)。
