摘要
目的 确定肺结核患者IgM激活C1q类双特异性循环免疫复合物 (IgM/C1q-TCIC)的水平并探讨其免疫生物学意义。方法 以羊IgG抗人C1q为包被抗体 ,HRP -羊IgG抗人IgM为指示抗体 ,建立捕捉法ELISA ,分别检测 12 7例肺结核、2 5例肺癌、2 7例肺炎和 4 31例正常人血清中IgM/C1q-TCIC。结果 肺部疾病按血清IgM/C1q-TCIC水平高低依次排序为肺结核 >肺癌 >肺炎。肺结核患者IgM/C1q-TCIC的阳性率与年龄、性别、Ig、C3 、ALT等其它临床指标无关 ,而与肺结核的初复治以及是否为活动性有关。结论 肺结核患者中IgM /C1q-TCIC是机体排除抗原的一种保护性反应 ,这种反应与机体的免疫反应性密切相关 ,可作为估价机体整体免疫反应的有效指标。
ObjectiveTo define the concentration and immunity biological significance of IgM and C 1q of two determined circulating immune complexes(IgM/C 1q -TCIC) in pulmonary tuberrculosis'(TB) patients.MethodsUsing goat IgG anti-human C 1q for coated antibody,HRP-goat IgG anti-human IgM for detected antibody.A capture ELISA method was established to detect IgM/C 1q -TCIC of 127 cases of TB,25 cases of lung cancer,27 cases of pneumonia.ResultsThe levels of serum IgM/C 1q -TCIC in patients with pneumonia disease placed in order were TB>lung cancer>pneumonia.The amount of IgM/C 1q -TCIC in TB patients had no relation to age,sex,Ig,C 3,ALT and the other clinical indicator,which was related to first or further treament and active or nonactive periods.ConclusionIgM/C 1q -TCIC in TB patients was an protecting reaction of eliminating antigens of bodies,which was closely related to immunity reactivity.IgM/C 1q -TCIC could be an useful index to evaluate the whole body immune reaction.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第12期1439-1440,共2页
Chinese Journal of Public Health
