摘要
目的 从形态学角度观察木瓜粉对体外培养鼠胚大脑神经细胞缺氧损伤预防效果的影响。方法 采用无血清体外细胞培养方法 ,培养鼠胚大脑神经细胞 ,放置含木瓜粉培养液的神经细胞于缺氧环境造成缺氧损伤 ,将细胞分为 4组 ,Ⅰ组、Ⅱ组、Ⅲ组为缺氧组 ,木瓜粉浓度分别为 0mg/ml、0 1mg/ml和 0 5mg/ml;Ⅳ组为非缺氧未加木瓜粉组 ,于细胞培养后的第 4天收集细胞 ,进行有关指标的观察测定。结果 Ⅱ、Ⅲ、Ⅳ组神经细胞DNA含量高于Ⅰ组 ,并有显著性差异 (P <0 0 5 )。光镜、电镜观察显示 ,Ⅰ组神经细胞数量少 ,外观残破 ,界限不清 ,突起较细疏短缺 ,可见凋亡小体 ;添加木瓜粉的Ⅱ、Ⅲ组多数细胞铺片良好 ,形态丰满 ,界限清楚 ,突起较粗壮 ,突起间网络比较稠密 ,核染色质虽有些凝聚 ,但未见凋亡小体。结论 木瓜粉能较好地预防鼠胚大脑神经细胞缺氧损伤。
ObjectiveTo observe the effects of papaya on the preventive efficacy of cerebral neurocytes following anoxic damage in vitro.MethodsThe rat embryonic cerebral neurocytes were cultured with serum-free medium which papaya was added in and were divided into four grous(group Ⅰ anoxic damage 0mg/ml,group Ⅱ anoxic damage 0.1mg/ml,group Ⅲ anoxic damage 0.5mg/ml and group Ⅳ non-anoxic damage 0mg/ml).At the end of the experiment,cultured cerebral neurocytes were collected to detect biochemical indexes and make a pathological observation by using electronic microscope.ResultsCompared with group Ⅰ,the level of DNA in group Ⅱ,Ⅲ and Ⅳ was increased significantly( P <0.05).The morphological observation showed that the number of neurocytes in group Ⅰ decreased,and the appearance of cell was broken,cell boundary was obscure,synapse were thin and short,and apoptosis bodies can be found.In group Ⅱ and Ⅲ,stretched preparation of majority cells was good,the body of neurocytes was full-grown,cell boundary was integrity,synapses were strong and network was dense,chromatin was partly condense,but apoptosis body can not be found.ConclusionPapaya can prevent the anoxic damage of rat cerebral neurocytes.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2002年第12期1422-1424,共3页
Chinese Journal of Public Health
