摘要
目的 探讨姜黄素对肝癌细胞(QGY)细胞增殖和细胞周期的调控及细胞凋亡的影响。方法 采用MTT法测定姜黄素在不同浓度、不同时间对QGY的抑制作用,流式细胞仪分析细胞周期分布,透射电镜观察细胞的超微结构变化。结果 姜黄素可抑制QGY细胞的生长,其抑瘤率与药物浓度和作用时间呈依赖关系,72h的中效浓度(IC50)为49.50μmol/L,流式细胞仪分析证实姜黄素能使QGY细胞聚积在S期,电镜观察发现姜黄素可导致细胞变性、坏死,诱导细胞凋亡。结论 姜黄素可干扰QGY细胞的周期分布,具有细胞毒作用、抗增殖、诱导细胞凋亡的作用。
Objective To investigate the effect of curcumin on the proliferation, cell cycle distribution and apopto-sis of hepatocarcinoma cell line QGY. Methods The MTT method was used to assay the biologic activities of curcumin in different times and different doses. The cell cycle distribution was detected by flow cytometic analysis. The cell ultrastruc-ture was observed by electronic microscopy. Results Curcumin could inhibit effectively QGY in a dose- and time- dependent manner. ICso of curcumin to QGY was 49.50 nmol/L in 72 hours. The cell growth was arrested at S stage. Curcumin could lead to the degeneration, necrosis, and apoptosis. Conclusions Curcumin can interrupt the cell cycle and has a role in cytotoxicity, antiproliferation and inducing apoptosis of QGY.
出处
《中华肝脏病杂志》
CAS
CSCD
2002年第6期449-451,共3页
Chinese Journal of Hepatology
