摘要
本文就MTT法在抗癌药物筛选试验中的应用方面进行了初步探讨,结果显示该方法简便、快速.细胞浓度以1—5×10~4/孔较为适宜,吸光度30min内不变.DMSO为MTT法的想理溶剂,但需除去蛋白质的影响.MTT以5mg/ml与细胞反应4h为佳.批内CV值为10%左右.四种抗癌药物对瘤株细胞SW-480作用后。
In this paper the procedure for the study of anticancer drug screenning on colon cancer cell line SW-480 using MTT (tetrazolium dye) assay has been observed in vitro. Our study showed that(l) The optimal target cell concentration was 1-5×10~4 cell/well. (2) The optimal MTT concentration is 5 mgml^(-1) when a range of 0-10 mgml^(-1) was tested. In view of the relatively small increase in sensitivity obtained after 6h, we chose 4h incubation as optiomal incubation time. (3) DMSO and acid isopropyl alcohol were both effective to dissolve the formazan produced but greater solubility and absorbance reading were seen using DMSO. (4) The addition of new born calf serum to the reaction mixture results in a gradual lowering of optical density than that produced by serum-free medium before the addition of DMSO.The assay is simple and rapid with a CV within groups about 10%. when using DMSO as a solvent in this assay, it is necessary to read the plates within 30 mim after adding DMSO. Meanwhile, the chemosensitivity of SW-480 cell to 72h exposrre to four kinds of standard anticancer drugs was different.
出处
《重庆医科大学学报》
CAS
CSCD
1991年第4期330-333,共4页
Journal of Chongqing Medical University
基金
卫生部资助课题
关键词
抗癌药
MTT法
试验
MTT Assay
Anticancer Drug Screenning
SW-480 Cell
