摘要
目的 探讨蛋白激酶A(cAMP -dependentproteinkinase ,PKA)在大鼠成骨细胞中的表达情况。方法 纯化培养大鼠成骨细胞后 ,以cAMP、PKI、rhBMP -2分别刺激细胞 3 0min ,设空白对照 ,通过免疫细胞化学及图象分析方法 ,观察细胞内PKA的活化表达情况。结果 各实验组均可见PKAα的活化表达 ,但是空白对照组表达弱(p <0 .0 5 ) ,PKI组最弱 (p <0 .0 1) ,cAMP组表达最强 (p <0 .0 1)。结论 在大鼠成骨细胞中存在功能状态的PKAα ,而且 ,rhBMP -2能激活PKAα。rhBMP
Objective To observe the expression and signal significance of protein kinase A on osteoblast cells in rats .Methods After being purified ,the cultured rat osteoblast cells were treated with cAMP , PKI , rhBMP-2 for 30 minutes . The expressions of PKAα were observed through immunocytochemical methods and image analysis. Results The expressions of PKAα were showed in cAMP, PKI, BMP and control group . But PKAα was weaker in control group( p < 0.05 ),weakest in PKI group( p < 0.01 )and strongest in cAMP group( p < 0.01 ).Conclusion PKAα is in functional status on osteoblast cells in rats. rhBMP-2 can activate PKAα.There is 'cross-talk' between BMP signal pathway and PKA signal pathway .
出处
《临床口腔医学杂志》
2002年第6期403-405,共3页
Journal of Clinical Stomatology
基金
国家自然科学基金 (30 0 70 81 8)
关键词
大鼠
成骨细胞
蛋白激酶A
免疫细胞化学
Osteoblast Cells
cAMP-dependent Protein Kinase
Immunocytochemistry
