摘要
体外培养成骨细胞，是研究力学环境，电磁刺激及药物治疗等理化因素对骨生长代谢影响机制的一种基本方法。如何消除或减少成纤维细胞的污染，鉴别纯化成骨细胞，是该方法应用中的主要困难。本实验中，观察了ＳＤ乳鼠顶骨组织学结构；建立起体外培养乳鼠顶骨成骨细胞模型；应用组织化学和免疫组织化学技术，对培养成骨细胞进行定性和定位，并将其纯化达９０％以上；形态学观察证实，成骨细胞在体外培养４０天左右时，有编织骨样骨组织形成，成骨细胞在体外培养中仍保持生骨能力。
AbstractIsolated bone eclls from the calvaria of neonatalSprague-Dawley rats were grown in RPMI 1640 and10%fetal bovine serum culture medium,For theidentification of osteoblastic cells from fibroblasticcells、the ALP immunohlstochemical technique wasapplied.Furification of the osteoblastic cells up to9O%was achieved by the technique of differentialadhesion and mechanical curetternent.The osteoblastsshowed a high ALP activity in the confluent state,which grew to form rnultiple layers and secreted richintercellular substance,The tudy demonstrated thatosteoblastic cells derived from rat calvaria have thecapacity to differerentiate into osteocytes and to formcalcified bone tissue in vitro.
出处
《中华骨科杂志》
CAS
CSCD
北大核心
1995年第6期364-366,共3页
Chinese Journal of Orthopaedics
基金
国家自然基金
