摘要
本研究介绍了一种简单、快速分析测定玉米中腐马素毒素(fumonisins)残留的方法。样品用甲醇+水混合液提取,对提取液用小型强酸型阴离子固相萃取柱(SAX)纯化。纯化后的样品经化学衍生处理后,用反相高效液相色谱分离,并以荧光分光光度检测器定量测定。该方法对于添加已知量、添加水平在0.3—2mg·kg-1腐马素的玉米样的测定证实:其平均回收率在80%以上,而该方法对于腐马素三种同系物的最低检出限分别为0.1—0.05mg·kg-1。此外,本方法也对有限数目的大米、大麦、小米、高粱样中存在腐马素毒素污染的可能性进行了验证,结论是否定的。
A simple, rapid procedure has been described for determination of fumonisins (FB1, FB2), the metabolites of Fusarium moniliforme and proliferatum in maize. The toxins were extracted by shaking after addition a mixture of methanol + water (3+1,V/V) as extractant and a mini-SAX solid phase column was employed for cleaning-up the sample. The toxins from cleaning up step were derivatized with O-phthaldialdehyde in the presence of mercaptoethanol as a catalyst to form highly fluorescent derivatives. A reversed phase LC was utilized for final separation of the sample and quantitation of the toxins was achieved by a fluorescent detection. The recoveries from fortified maize samples were found to be 78.0%— 95.7% with relative variation of 5.2%— 12.7 %, respectively. The minimum detection limits of the present method for FB1 and FB2 were 0.04 and 0.1 mg·kg-1, respectively.
出处
《农业环境保护》
CSCD
北大核心
2002年第6期512-515,共4页
Agro-Environmental Protection
基金
科技部2000年公益研究资助项目
