摘要
目的 研究在长期培养大鼠海马脑片各区中N -甲基 -D -门冬氨酸 (N -methyl-D -aspartate,NMDA)受体亚单位NR2A、NR2B表达的变化规律。方法 4 0 0 μm厚大鼠海马脑片 ,体外培养 1、2、3、4、5周 ,再作 2 0 μm厚冰冻切片 ,行NR2A、NR2B免疫组织化学反应和图像分析。结果 NR2A、NR2B在培养各周海马脑片各区锥体细胞和颗粒细胞胞体均有表达 ,NR2B还在CA1区锥体细胞顶树突明显着色。 1周时 ,NR2A的表达较弱 ;4周时升至高峰。在CA3区 ,NR2B的表达 1周时即达高峰 ;但在CA1区其表达至 3周时始达高峰。结论 长期培养大鼠海马脑片各区中NR2A、NR2B的表达有时空变化 ,且二者的表达模式不同 。
Objective To probe into the expressions of NMDA receptor subunits 2A and 2B (NR2A and NR2B) in long-term hippocampal organotypic tissue cultures (HOTCs) of rat.?Methods The 400 μm thick hippocampal slices of SD rat pups were cultured separately for 1, 2, 3, 4, 5 weeks in vitro (wiv). The cultured slices were freezing sectioned in 20 μm thickness to study the immunohistochemical reactions of NR1, which were performed by ABC method and evaluated by LEICA QWin image analysis system.?Results NR2A and NR2B were expressed in the body of all pyramidal cells and granular cells in rat HOTCs at every wiv, and NR2B was also obviously expressed in the apical dendrites of pyramidal cells in CA1 field. The expression of NR2A was relatively weak at 1 wiv and reached its peak at 4 wiv. The expression of NR2B attained its peak at 1 wiv in CA3, but at 3 wiv in CA1.?Conclusion The expressions of NR2A and NR2B in long-term HOTCs of rats are of time-space dependent features and are different in mode. This suggests that the structure and function of NR2A and NR2B may also be varying in long-term HOTCs.
出处
《徐州医学院学报》
CAS
2002年第6期474-478,共5页
Acta Academiae Medicinae Xuzhou
基金
江苏省自然科学基金项目 (K99192 )
江苏省麻醉学重点实验室开放课题(K990 37)
江苏省重点学科徐州医学院生物化学与分子生物学研究中心开放课题 (990 0 1)
