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离体培养神经干细胞中NMDA受体亚单位NR2B的表达 被引量：5

The expression of NMDA receptor subunit 2B in neural stem cells in vitro
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摘要 目的研究离体培养的大鼠神经干细胞（NSCs）中N-甲基-D-天冬氨酸（NMDA）受体亚单位NR2B的表达。方法从孕18～19天胎鼠海马中分离、培养、传代、鉴定NSCs，用免疫细胞化学、Western blot免疫印迹检测传代1次NSCs中NMDA受体亚单位NR2B的表达。结果源自孕18～19天胎鼠海马的NSCs，即可以表达NMDA受体亚单位NR2B。结论体外培养的NSCs能表达NMDA受体亚单位NR2B。 Objective To investigate the expression of NMDA receptor subunit 2B in the cultured neural stem cells （NSCs）. Methods NSCs from the hippocampus of rats at embryonic day 18 - 19 were isolated, cultured, passaged and identified. The expression of N- methyl- D - aspartate （NMDA） receptor subunit 2B in the passaged NSCs was determined by immunocytochemistry and Western blot. Results It was found that NSCs derived from the hippocampus of rats at embryonic day 18 - 19 could express NMDA receptor subunit 2B. Conclusion NSCs can express NMDA receptor subunit 2B in vitro.
作者 胡忠浩 王珊珊 徐铁军
机构地区 徐州医学院人体解剖和神经生物学教研室
出处 《徐州医学院学报》 CAS 2006年第4期286-289,共4页 Acta Academiae Medicinae Xuzhou
基金 江苏省高等学校研究生创新计划资助项目 徐州医学院留学归国人员科研启动基金资助项目
关键词 神经干细胞 NMDA受体亚单位2B WESTERN BLOT neural stem cells NMDA receptor subunit 2B Western blot
分类号 Q189 [生物学—神经生物学]
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参考文献14

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徐州医学院学报
2006年 第4期

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