摘要
目的 探讨环氧化酶 2 (COX 2 )mRNA在乳腺癌中的表达以及与乳腺癌临床病理特征的关系。方法 以 β actin基因为参照 ,应用逆转录聚合酶链反应 (RT PCR)技术 ,检测 30例乳腺癌组织及癌旁正常腺体中COX 2mRNA的表达。应用条带密度分析软件 ,定量分析RT PCR产物电泳带密度。结果 30例癌组织中 ,2 8例COX 2mRNA表达水平明显增高 ,全距为 0 .0 5～ 0 .91,中位数为 0 .5 3;癌旁正常腺体中无表达或表达很弱 ,全距为 0～ 0 .0 9,中位数为 0。癌组织与正常腺体中COX 2的表达差异有显著性 (P <0 .0 5 )。COX 2的高表达与淋巴结的转移有关 (P <0 .0 5 ) ,而与年龄、癌肿的大小、临床病理分期、病理类型无关 (P >0 .0 5 )。结论 乳腺癌组织中COX 2mRNA表达水平高于正常腺体 ,其表达与乳腺癌的发生、发展及淋巴结转移有关。
Objective To study the expression of cyclooxygenase-2(COX-2) gene in breast cancer and its clinicopathologic characteristics. Methods With β-actin gene as reference, the COX-2 mRNA was monitored in 30 specimens of breast cancer tissue and adjacent normal breast tissue by reverse transcription-polymerase chain reaction (RT-PCR). Results The COX-2 mRNA expression was significantly upregulated in most breast cancer tissues with range of 0.05-0.91 (median 0.56), which was rare in normal breast tissue with range of 0-0.09 (median 0). The difference of COX-2 mRNA expression between cancer and normal breast tissue was significant(rank sum test, P<0.05). COX-2 overexpression in breast cancer was related to its lymph node metastasis(P<0.05) but not to age, tumor size, pathologic grade or pathologic type (P>0.05).Conclusion The level of COX-2 mRNA expression is obviously higher in the breast cancer tissue than that in normal breast tissue. COX-2 overexpression may play a crucial role in the carcinogenesis, development of cancer and lymph node metastasis in breast cancer patients.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2002年第6期567-569,共3页
Chinese Journal of Oncology
