摘要
【目的】了解常见的皮肤肿瘤组织环氧合酶-2(COX-2)和凋亡相关基因BCL-2表达的情况及其临床意义,初步探讨COX-2与BCL-2在皮肤肿瘤中过表达对肿瘤形成和发展的作用。【方法】免疫组织化学法检测脂溢性角化病(SK)15例,Bowen’s病(BD)15例,基底细胞上皮瘤(BCE)20例,鳞癌(SCC)20例及正常组织5例COX-2和BCL-2蛋白表达。【结果】各标本的肿瘤组织均有COX-2的表达,总体上的阳性率无显著性差异(χ2=0.148,P>0.05),染色强度有显著性差异(Hc=90.70,P<0.05),周围正常组织未见COX-2的表达。SCC、BD表达范围弥漫,以SCC表达强度最为显著;细胞分化高的SCC标本其COX-2的表达较细胞分化低者明显。BCE、SK呈灶性表达,强度不一;正常组织仅于表皮基底层有强度很弱的阳性表达。在SCC、BCE、BD,COX-2表达阳性者BCL-2表达的阳性率较COX-2表达阴性者高(P<0.05)。【结论】皮肤肿瘤存在COX-2的过表达;检测SCC组织COX-2的表达可预测其病变的恶性程度;COX-2表达的模式较阳性与否、表达的强度对病变的提示更有意义。BCL-2的表达与COX-2的过表达有关,COX-2可能是通过激活BCL-2发挥抗凋亡作用而促进皮肤肿瘤形成及发展。
[Objective] To investigate the expression of cyclooxygase-2 protein （COX-2） and apoptosis associated gene bcl-2 protein in skin neoplasms and primarily explore their over-expression in formation and development of neoplasms. [Methods] Immunohistochemical staining was applied to detect COX-2 and BCL-2 protein expression in 15 cases of seborrheic keratosis （SK）, 15 Bowen＇s disease （BD）, 20 basal cell epithelioma （BCE）, 20 squamous-cell carcinoma （SCC） and 5 normal skin tissues. [Results] Four kinds of neoplasms showed COX-2 expression. Surrounding normal tissues did not show COX-2 expression. There was no significant distinction in the general positive rate （x^2=0.148,P 〉0.05）; staining intensity displayed significant distinction （Hc=90.70,P〈 0.05）. Expression of SCC and BD was diffused; SCC proved the most remarkable expression density. Higher cellular differentiation SCC specimen was more intense than lower ones. Positive staining intensity was diverse in BCE and SK. Normal tissues specimen manifested a weak staining in the basal layer. Positive Bcl-2 expression was higher in COX-2 positive expression of SCC, BCE, BD than those in negative COX-2 group.[Conclusion] COX-2 overexpression exists in skin neoplasm. Detection of COX-2 expression in SCC can used to estimate malignancy degree and cellular differentiation. Pattern of COX-2 staining means more than intensity and positive reaction. BCL-2 expression was correlated with over-expression of COX-2. COX-2 may promote skin neoplasm formation and development by stimulating anti-apoptosis of BCL-2 protein.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
北大核心
2006年第2期169-172,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
广东省医学科研基金资助项目(B2005032)
