摘要
介绍了一种利用噬菌体肽库的新技术———体内噬菌体展示(invivophagedisplay)。这项技术是在活的动物体内进行的肽库筛选,将肽库通过静脉注射到动物体内,因为血管分子内皮的异质性,噬菌体可以选择性地导向不同组织,这样就可以筛到与特定组织特异结合的噬菌体展示肽。动物实验表明,前凋亡小肽和细胞毒素与导向肽偶联后提高了治疗效果。这项技术应用于临床,一定有助于肿瘤等疾病的导向治疗和造影技术的发展。
Pasqualini et al.has developed an in vivo selection system in which phage capable of selective homing to different tissues are recoved from a phage display peptide library following intravenous administration.The peptide that result from such a selection home to specific organs and tissues because they recognize molecular addresses,re-ceptors that are differentially expressed in viscular beds.Targeting delivery of chemotherapeutics,pro-apoptotic pep-tide and cytokines to tumors using these peptides improved therapeutic efficacy in animal models.Translation of this technology into clinical applications will form the basis for targeting therapeutic and imaging agents in the context of cancer and other diseases.
出处
《生物技术通讯》
CAS
2002年第6期462-465,共4页
Letters in Biotechnology
