摘要
以龙眼胚性愈伤组织为起始材料 ,研究了胚性悬浮细胞培养的影响因素、继代保持方法及其体胚发生与植株再生 .结果表明 ,建立龙眼优良胚性悬浮细胞系的技术关键在于 :由松散型胚性愈伤组织作为起始材料 ;以去除琼脂的保持松散型胚性愈伤组织的培养基作为启动培养基 ;采用分别附加 5mgL-1AgNO3 、10 0mgL-1肌醇的培养基交替继代保持 .胚性悬浮细胞在附加 2 %蔗糖、0 .7%琼脂、4 0 0mgL-1LH、10 0mgL-1肌醇的MS固体培养基上形成的体胚数量可达 7× 10 4～ 14× 10 4g-1.经过成熟培养后 ,体胚转换植株的频率在 6 5 %以上 .图版 1图 1表 3参
The factors affecting establishment of embryogenic suspensions from embryogenic calli, the methods of suspension maintenance and plant regeneration via somatic embryogenesis of longan were studied. The results showed that the key factors for establishing an excellent embryogenic suspension were as follows: initiating from friable embryogenic callus in the liquid medium with the same components as the subculture medium of friable embryogenic callus free of agar; maintaining suspensions via alternative culture of the media amended with 5 mg L -1 AgNO 3 and 100 mg L -1 myo inositol, respectively. The embryogenic suspension cells formed somatic embryos at the frequency of 70 000～140 000 g -1 on the solid MS medium amended with 2% sucrose, 0.7% agar, 400 mg L -1 LH and 100 mg L -1 myo inositol. The somatic embryos were converted into plantlets at the frequency of over 65% after maturation. Plate 1, Fig 1, Tab 3, Ref 6
出处
《应用与环境生物学报》
CAS
CSCD
2002年第5期485-491,共7页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金 (39870 544)
国家教育部首批高等学校骨干教师资助计划
霍英东教育基金会高等学校青年教师基金 (71 0 2 6)
福建省自然科学基金 (B0 0 1 0 0 1 4 )等资助项目的部分内容~~
关键词
龙眼
胚性细胞
悬浮培养
再生植株
longan
embryogenic suspension
plant regeneration
somatic embryogenesis
